H2662

Anti-Histone Deacetylase 7 (HDAC7) (KG-17) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Sinónimos: Anti-HD7, Anti-HD7A, Anti-HDAC7A
• Número MDL: MFCD04040364
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• conjugado: unconjugated
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• Formulario: buffered aqueous solution
• mol peso: antigen ~105 kDa
• reactividad de especies: mouse, human, rat
• técnicas: immunoprecipitation (IP): 1.0-1.5 μg using RIPA extract of 293T cells expressing recombinant human HDAC7; indirect immunofluorescence: 1:50 using rat NRK cells; western blot: 1:1,000 using whole extracts of mouse NIH-3T3 cells
• Nº de acceso UniProt: Q8WUI4
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... HDAC7(51564) ; mouse ... Hdac7(56233) ; rat ... Hdac7a(84582)

Descripción

Descripción general

Mammalian histone deacetylases (HDACs) can be divided into three classes according to sequence homology. Class I consists of the yeast transcriptional regulatory protein Rpd3-like proteins HDAC1, HDAC2, HDAC3, and HDAC8. Class II consists of the yeast Hda1-like proteins HDAC4, HDAC5, HDAC6, HDAC7, HDAC9 and HDAC10. Class III consists of the yeast sirtuin 2 (Sir2)-like proteins. Although HDAC7 is localized mostly to the cell nucleus, it is also found in the cytoplasm.

Especificidad

Anti-Histone Deacetylase 7 recognizes human, mouse, and rat HDAC7.

Inmunógeno

Synthetic peptide corresponding to amino acids of human HDAC7, conjugated to KLH.

Aplicación

Anti-Histone Deacetylase 7 (HDAC7) (KG-17) antibody produced in rabbit has been used in

• indirect immunofluorescene
• immunoblotting
• immunoprecipitation

Acciones bioquímicas o fisiológicas

Class II histone deacetylases (HDACs) have been implicated in the regulation of muscle differentiation. Interaction of HDAC4, -5, and -7 with members of the myocyte enhancer factor-2 (Mef2) family of transcription factors represses their transcriptional activity and prevents myogenesis. Shuttling of HDAC7 between the cell nucleus and the cytoplasm is controlled by a mechanism involving calmodulin independent kinase I (CaMKI) and 14-3-3 proteins. The HDAC7 enzymatic activity depends on its interaction with the class I HDAC3, and the corepressors silencing mediator of retinoic acid and thyroid hormone receptors (SMRT) and nuclear receptor co-repressor (N-COR). HDAC7 also interacts with the transcriptional repressor B-cell lymphoma 6 (BCL-6).

Forma física

Solution in 0.01 M phosphate buffered saline containing 1% bovine serum albumin and 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): nwg
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable