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GE17-5166-01

Sigma-Aldrich

Mono Q® 5/50 GL

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

ligand

-OCH2CH(OH)CH2OCH2CH(OH)CH2N+(CH3)3

material

PEEK column

description

Ion Exchanger Type (value)

packaging

pkg of 1 ea

parameter

580 psi

bed volume

1 mL

column I.D.

5.7 mm

matrix

monodisperse porous polystyrene/divinyl benzene beads
polymeric

matrix active group

ammonium ion, quaternary phase

avg. part. size

10 μm

cleaning

1-14

working range

2-12

separation technique

anion exchange

General description

Mono Q® is a strong anion exchanger prepacked with MonoBeads in a Tricorncolumn. The column is an excellent choice for small-scale polishing in purification of proteins, peptides, and other biomolecules when high purity is required.

The Tricorn columns offer high-resolution separations with greater loading capacity but slightly lower resolution than MiniBeads columns.

Features and Benefits

  • Monodisperse 10 μm porous beads yield high resolution, dynamic capacity, reproducibility, and durability.
  • Maximum resolution is achieved from the efficiency of small, perfectly spherical monodispersed particles optimally packed in columns coupled with the excellent selectivities of the Q ion exchangers.
  • Compatible with AKTA design, FPLC™ System, and other high-performance LC systems.
  • MonoBeads are also available as Mono P® for chromatofocusing

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

FPLC is a trademark of Cytiva
Mono P is a registered trademark of Cytiva
Mono Q is a registered trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Storage Class

3 - Flammable liquids

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Certificados de análisis (COA)

Lot/Batch Number

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Artículos

Performing an Isolation of Recombinant Protein Complexes

This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from Cytiva.

Optimizing Purification of Histidine-Tagged Proteins

How to optimize purification of histidine-tagged proteins using Cytiva products.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

Ver todo

Protocolos

MonoBeads: Purification of Milligram Quantities with Highest Resolution

This page covers using Monobeads for purification of proteins, peptides, or oligonucleotides.

Affinity Chromatography in a Purification Strategy (CIPP)

This page shows the purification strategy of capture, intermediate purification, and polishing (CIPP) in affinity chromatography using Cytiva products.

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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