GE17-0920-07
IMAC Sepharose™ High Performance
Cytiva 17-0920-07, pack of 100 mL
About This Item
Productos recomendados
packaging
pack of 100 mL
manufacturer/tradename
Cytiva 17-0920-07
matrix
highly cross-linked 6% agarose
avg. part. size
34 μm
cleaning in place
2-14(Medium uncharged with metal ions.)
working range
3-12
capacity
≥40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)
General description
Application
Features and Benefits
- For optimizing purification of histidine-tagged Proteins when Ni2+ is not the best choice of metal ion
- Conveniently charge with your metal of choice
- 34 μm bead size for high performance purification
- High binding capacity
Storage and Stability
Analysis Note
Legal Information
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Artículos
How to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography.
This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from Cytiva.
Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.
How to optimize purification of histidine-tagged proteins using Cytiva products.
Protocolos
Protocol for Purification of Histidine-Tagged Proteins using IMAC Sepharose® High Performance
Guide for separating poly(His) fusion proteins using affinity chromatography tags in various purification products.
Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules
This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.
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