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G9382

Sigma-Aldrich

Gelatin from bovine skin

gel strength ~225 g Bloom, Type B

Sinónimos:

Bovine gelatin

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About This Item

Número de CAS:
9000-70-8
EC Number:
232-554-6
MDL number:
MFCD00081638
UNSPSC Code:
12352202
NACRES:
NA.26
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biological source

bovine skin

Quality Level

200

type

Type B

assay

≥95% protein basis (biuret)

form

powder

technique(s)

ELISA: suitable
cell culture | mammalian: suitable
immunocytochemistry: suitable
western blot: suitable

solubility

H2O: soluble 50 mg/mL

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Components

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue. Type B is derived from lime-cured tissue.

Caution

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

Preparation Note

This product is derived from bovine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. This product has a gel strength of 225.

Storage Class

11 - Combustible Solids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000414230
0000414231
0000414231
0000414230
0000406718

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Ochuko L Erukainure et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 83, 1478-1484 (2016-10-25)
The immunomodulatory potentials of the crude methanolic extract and fractions [n-hexane (Hex), n-dichloromethane (DCM), ethyl acetate (EtOAc) and n-butanol (BuOH)] of Clerodendrum volubile flowers were investigated on whole blood phagocytic oxidative burst using luminol-amplified chemiluminescence technique. They were also investigated
T H Kim et al.
Hepatology (Baltimore, Md.), 31(1), 75-82 (1999-12-29)
Partial hepatectomy triggers a variety of biological phenomena, which culminate in regeneration of the liver mass. Hepatocyte proliferation is a major feature of the regenerating liver after partial hepatectomy. Previous studies in our laboratory suggested that hepatic matrix remodeling might
Isolation and maintenance of symbiotic fungi of ants in the tribe Attini (Hymenoptera: Formicidae)
Silva-Pinhati A C O, et al.
Neotropical Entomology, 34, 1-5 (2005)
Substrate specificity of thermostable alkaline protease from Bacillus sp. No. AH-101.
H Takami et al.
Bioscience, biotechnology, and biochemistry, 56(2), 333-334 (1992-02-01)
G Alessandri et al.
Cancer research, 43(4), 1790-1797 (1983-04-01)
An assay to measure endothelial cell mobilization on a gelatin substratum has been developed. Utilization of the gelatin-agarose and Boyden chamber assays established that: (a) fragments or extracts of corneas treated with several effectors of angiogenesis in vivo acquired the
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Questions

1–10 of 10 Questions  

  1. What is the molecular weight of this Gelatin?

    1 answer

    1. The molecular weight of this product has not been evaluated, However, the molecular weight is closely related to the Bloom Number. The estimated ranges are as follows:
      Bloom Number Average Molecular Mass (Da)
      50−125 (Low Bloom) 20,000−25,000
      175−225 (Medium Bloom) 40,000−50,000
      225−325 (High Bloom) 50,000−100,000

      Please see the link below to review the general datasheet for Gelatin:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/333/625/g9382pis.pdf

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  2. How to sterilize 2% solution?

    1 answer

    1. This item can be sterilized by autoclaving at 121°C, 15 psi for 30 minutes.

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  3. Which is the molecular weight?

    1 answer

    1. The Bloom number or gel strength of this product is ~225 g Bloom. The average molecular mass of 175-225 g Bloom (Medium Bloom) products is 40,000-50,000 Da. The average molecular mass of 225-325 g Bloom (High Bloom) products is 50,000-100,000 Da. Therefore, this product is expected to have a molecular weight of approximately 50 kDa.

      For more information please view the product data sheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/187/614/g6650-et-al-pis-ms.pdf

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  4. Is this made from skin from a cow which was killed for the food chain?

    1 answer

    1. All information that is shared regarding source can be found on a lot-specific Certificate of Origin (CofO, COO) for this product.

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  5. What is a Bloom number, and how does it correlate with the molecular weight?

    1 answer

    1. The Bloom number is indicative of gel strength. The Bloom number is the amount of pressure, in grams, required to break the surface of a 6.67% gel. The general rule of thumb in regards to an average molecular weight: High Bloom (225-325) = 50,000-100,000; Medium Bloom (175-225) = 40,000-50,000; Low Bloom (50-125) = 20,000-25,000. Product G9382 is a high Bloom preparation of approximately 225 Bloom.

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  6. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  7. What is Product G9382, Gelatin from bovine skin, soluble in?

    1 answer

    1. Gelatin is soluble in glycerol and acetic acid, and more soluble in hot water than in cold water.  We test the solubility of gelatin in water at 50 mg/mL (with heat).

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  8. What is the isoelectric point (pI) for Product G9382, Gelatin from bovine skin?

    1 answer

    1. The pI for Product No. G9382 is typically in the range of 4.7 - 5.0.

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  9. How does the storage temperature relate to shipping conditions?

    1 answer

    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

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  10. What is the difference between type A and type B gelatin?

    1 answer

    1. Type A gelatin is derived from acid-cured tissue whereas Type B gelatin is derived from lime-cured tissue. Type A gelatin has about 78-80 millimoles of free carboxyl groups per 100 g of protein and a pI of 7.9-9.0. Type B has 100-115 millimoles of free carboxyl groups per 100 g of protein and a pI of 4.7-5.2.

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