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Merck

G7400

Sigma-Aldrich

Galactose Oxidase from Dactylium dendroides

lyophilized powder, ≥3,000 units/g solid

Sinónimos:

D-Galactose:oxygen 6-oxidoreductase

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MXP 2,483.00
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Fecha estimada de envío15 de abril de 2025



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1000 UNITS
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About This Item

Número de CAS:
Comisión internacional de enzimas:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

MXP 2,483.00


Fecha estimada de envío15 de abril de 2025


biological source

fungus (Dactylium dendroides)

Quality Level

form

lyophilized powder

specific activity

≥3,000 units/g solid

storage temp.

−20°C

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General description

Galactose oxidase is an extracellular copper-containing enzyme, secreted by the deuteromycete fungus Dactylium dendroides. It catalyzes the oxidation of a range of primary alcohols, including D-galactose, to the corresponding aldehyde, with reduction of oxygen to hydrogen peroxide.[1][2]

Application

Galactose oxidase may be used as an analytical tool for the specific determination of D-galactose in blood plasma, plant extracts, and phospholipids. It could be used for the characterization of terminal D-galactoside units in several polymers.[3]

Biochem/physiol Actions

Galactose oxidase catalyzes the coversion of D-galactose to D-galacto-hexodialdose.
2-Deoxy-D-galactose, lactose, melibiose, raffinose and stachyose react with galactose oxidase in the peroxidase:o-tolidine system.
Essentially no oxidation of D-glucose, L-galactose, L-arabinose or D-glucuronate has been observed.

Unit Definition

One unit will produce a ΔA425 of 1.0 per min at pH 6.0 at 25 °C, in a peroxidase and o-tolidine system. Reaction volume = 3.4 mL. Light path = 1 cm.

Related product

inhibitor

Referencia del producto
Descripción
Precios

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Yuling Sun et al.
Biomacromolecules, 22(6), 2595-2603 (2021-05-07)
Antifreeze glycoproteins (AFGPs) are able to bind to ice, halt its growth, and are the most potent inhibitors of ice recrystallization known. The structural basis for AFGP's unique properties remains largely elusive. Here we determined the antifreeze activities of AFGP
Bei Yue et al.
Frontiers in pharmacology, 12, 774560-774560 (2021-11-20)
Irinotecan (CPT11), a broad-spectrum cytotoxic anticancer agent, induces a series of toxic side-effects. The most conspicuous side-effect is gastrointestinal mucositis, including nausea, vomiting, and diarrhea. A growing body of evidence indicates that bacteria β-glucuronidase (GUS), an enzyme expressed by intestinal
A R Shatzman et al.
Journal of bacteriology, 130(1), 455-463 (1977-04-01)
The effects of pH and growth density on the amount of an extracellular enzyme, galactose oxidase, synthesized by the fungus Dactylium dendroides were studied. Growth at a pH below 6.7 caused a decrease in the ability of the organism to
Biocatalytic desymmetrization of an atropisomer with both an enantioselective oxidase and ketoreductases.
Bo Yuan et al.
Angewandte Chemie (International ed. in English), 49(39), 7010-7013 (2010-08-18)
Development of a galactose biosensor with galactose oxidase-immobilized epidermis of Solanum lycopersicum: potential point-of-care testing for citrin deficiency in high-prevalence areas.
Hencher H C Lee et al.
Clinica chimica acta; international journal of clinical chemistry, 412(3-4), 391-392 (2010-10-26)

Questions

  1. Hello, I have bought this enzyme for some assays in our lab in January 2024. I would like to ask how this enzyme is produced? Is it recombinant (E. coli or yeast) or is it directly isolated from Dactilium dendroides? Looking forward to hearing from you!

    1 answer
    1. This enzyme is from D. Dendroides and is recombinantly produced from Yeast in an animal component free fermentation process.

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