G100120
Sephadex® G-100
Medium
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About This Item
Productos recomendados
form
slurry
technique(s)
LPLC: suitable
matrix
Agarose
matrix active group
polymer
swelling
1 g swells to 15-20 mL gel
bead size
40-120 μm
separation technique
size exclusion (SEC)
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General description
Sephadex® is composed of repeating glucose units attached by α-1,6 glucosidic bonds. This structure ensures more aptamer binding sites and greater binding capacity. It is suitable for large-scale purification and is considered stable and can be utilized more than once.
Fractionation Range (MW)
Globular Proteins: 4,000 - 150,000
Dextrans: 1,000 - 100,000
Fractionation Range (MW)
Globular Proteins: 4,000 - 150,000
Dextrans: 1,000 - 100,000
Application
Sephadex®G-100 is a gel filtration medium used in protein chromatography, affinity chromatography and gel filtration chromatography
It has been used:
It has been used:
- in gel filtration column for α-amylase purification
- in gel filtration column for the purification of polysaccharidic fraction extracted from the skin of the ray Raja radula
- to remove excess protamine by size exclusion from cross-linked iron oxide (CLIO)-protamine-linked (B) peptides
Other Notes
G100120-100G′s updated product number is GE17-0060-01
Legal Information
Sephadex is a registered trademark of Cytiva
replaced by
Referencia del producto
Descripción
Precios
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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AmyLa α-amylase gene from Laceyella sp. DS3 was heterologously expressed in E. coli BL21. E. coli BL21 maximally expressed AmyLa after 4 h of adding 0.02 mM IPTG at 37 °C. The recombinant AmyLa α-amylase was purified 2.19-fold through gel filtration and ion
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The polysaccharide fraction from the skin of the ray Raja radula was extracted, characterized and assayed for anticoagulant activity. A whole polysaccharidic fraction was extracted from the skin of the ray Raja radula by papain digestion followed by cetylpyridinium chloride
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Production, purification and characterization of an a-amylase produced by <I>Saccharomycopsis fibuligera</I>
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