E8156

Anti-eRF1 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Sinónimos: Anti-Eukaryotic translation termination factor 1, Anti-RF1, Anti-Release factor 1, Anti-eTF1
• UNSPSC Code: 12352203
• NACRES: NA.41

Propiedades

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 50 kDa
• species reactivity: rat, human, mouse
• packaging: antibody small pack of 25 μL
• concentration: ~1 mg/mL
• technique(s): immunoprecipitation (IP): 1-2 μg using HeLa cell lysates.; western blot: 0.5-1 μg/mL using 3T3 or Rat1 cell extracts.
• UniProt accession no.: P62495
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ETF1(2107) ; mouse ... Etf1(225363) ; rat ... Etf1(307503)

Descripción

General description

Eukaryotic translation termination factor 1 (eRF1) is a protein encoded by the ETF1 gene in humans. It belongs to eRF1 family and the amino-acid sequence is highly conserved.

Application

Anti-eRF1 antibody produced in rabbit has been used in western blotting and immunoprecipitation.

Biochem/physiol Actions

Eukaryotic translation termination factor 1 (eRF1) proteins are directly implicated in the termination of translation in eukaryotes. It plays an important role in the termination of protein synthesis, which is performed by three domains (N, M and C) of eRF1. Rearrangement in eRF1 leads to N-domain accommodation into the ribosomal A site.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)