In Volume 2 of Essential Molecular Biology, procedures for preparing gene libraries and identifying genes are described, together with methods for studying the structure of a cloned gene and the way it is expressed in the cell.
Índice
1. Strategies for research in molecular biology, T. A. Brown 2. Construction of genomic libraries in lambda and cosmic vectors, Anna-Mariea Frischauf 3. Construction of a cDNA library, Madan K. Bhattacharyya 4. Nucleic acid labeling and detection, C. R. Mundy et al. 5. Immobilization of nucleic acids and hybridization analysis, N. J. Dyson 6. DNA sequencing, T. A. Brown 7. The Polymerace chain reaction, T. A. Brown 8. Protien expression in Escherichia coli, John M. Ward 9. Transcript mapping, Colin P. Smith Appendices Health hazards and safety procedures Equipment for molecular biology research Important Escherichia coli strains Recipes and general procedures Restriction endonucleases DNA and RNA modification enzymes List of suppliers
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