D4138

Deoxyribonuclease II from porcine spleen

Type IV, lyophilized powder, 2,000-6,000 Kunitz units/mg protein (biuret)

• Sinónimos: DNase II, Deoxyribonucleate 3′-oligonucleotido-hydrolase
• Número de CAS: 9025-64-3
• Comisión internacional de enzimas: 3.1.22.1 (BRENDA, IUBMB)
• Número CE: 232-801-8
• Número MDL: MFCD00130919
• Código UNSPSC: 12352204
• NACRES: NA.54

Propiedades

• tpo: Type IV
• Formulario: lyophilized powder
• actividad específica: 2,000-6,000 Kunitz units/mg protein (biuret)
• mol peso: 45 kDa
• composición: Protein, 60-80%
• actividad extraña: RNase ≤0.05%
• temp. de almacenamiento: −20°C

Descripción

Aplicación

DNase II from Sigma has been used to treat transformed cells during the purification of β-lactamase.[1] It has also been used for the preparation of adenoma tissue in a study that investigated the effect of somatoprim on growth hormone secretion in human adenoma cell cultures (hSA).[2]

Deoxyribonuclease II from porcine spleen has been used in an immunohistological study of the immune system cells in paraffin-embedded tissues. Deoxyribonuclease II from porcine spleen has also been used in a study to investigate its reassociation with the lysosomal membrane.

Acciones bioquímicas o fisiológicas

The enzyme is a lysosomal acid hydrolase that hydrolyzes DNA to 3′-phosphoryl oligonucleotides in the absence of metal ions, under acidic conditions.[3] It is a non-covalently linked α·β heterodimer of approximately 45,000 Da molecular mass.[4]

Definición de unidad

One Kunitz unit will produce a ΔA₂₆₀ of 0.001 per min per mL at pH 4.6 at 25°C; [Mg²⁺] = 0.83 mM

Forma física

Contains sodium chloride

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable