Looking on the datasheet, it states that there is 250 ug of DNA per vial and that the vial has 100 x 5uL loads (500 uL volume). DNA concentration is 0.5 ug/uL. Loading 5 uL per well therefore provides a DNA load of 2.5 ug per well. Please view the data sheet for more information: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/157/148/d3937dat.pdf
D3937
DirectLoad™ 1 kb DNA Ladder
ready-to-use marker for DNA electrophoresis
Sinónimos:
1 kb marker, 1kb gel ladder, 1kb gel marker, 1kb ladder for gel electrophoresis, 1kb marker for gel electrophoresis, DNA ladder, DNA marker, DirectLoad™ marker, RTU DNA ladder, RTU DNA marker, agarose gel electrophoresis ladder, agarose gel electrophoresis marker, ready to use DNA ladder, ready to use DNA marker
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1 VIAL
MXP 5,502.00
MXP 5,502.00
Fecha estimada de envío30 de marzo de 2025
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1 VIAL
MXP 5,502.00
About This Item
UNSPSC Code:
41105335
NACRES:
NA.25
MXP 5,502.00
Fecha estimada de envío30 de marzo de 2025
Productos recomendados
Quality Level
form
liquid
usage
100 uses
suitability
suitable for electrophoresis (DNA)
storage temp.
−20°C
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General description
Sigma′s DirectLoad™ 1 kb Ladder contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb, and 2 kb repeats from 6 to 10 kb. For NA electrophoresis, 5 ul of the marker can be loaded directly into a single lane on an agarose or polyacrylamide gel. Suitable for use in Northern and Southern blotting.
Application
Suitable for size determination of dsDNA by DNA electrophoresis with either agarose or polyacrylamide gels.
Features and Benefits
- Ready-to-load
- Easy-to-use
- Popular band sizes
Components
Sigma′s DirectLoad™ 1kb Ladder is provided in a gel-loading solution of 2.5% Ficoll (Type 400), 0.0125% bromophenol blue, and 0.00625% xylene cyanol.
Other Notes
Contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb and 2 kb repeats from 6 to 10 kb.
For optimal resolution, the recommended agarose gel concentration is 0.75%.
Legal Information
DirectLoad is a trademark of Sigma-Aldrich Co. LLC
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Referencia del producto
Descripción
Precios
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Artículos
Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.
Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.
Protocolos
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.
Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
JumpStart™ Taq DNA Polymerase is an antibody-inactivated, hot start enzyme.
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