Both D2438 and D2650 are suitable for cell culture. The D2650 is part of the Hybri-Max™ product line. Hybri-Max products have been tested for use in hybridoma creation and culture. The D2438 is part of the BioPerformance Certified group of products. These products are tested and optimized for use in biological applications. For this product and a number of others, this includes testing to meet USP and EP guidelines. Please see the links below to review a sample Certificate of Analysis for each item.
D2650
https://www.sigmaaldrich.com/product/sigma/d2438#product-documentation
D2438
https://www.sigmaaldrich.com/product/sigma/d2650#product-documentation
D2438
Dimetilsulfóxido
Dimethyl sulfoxide
sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma
Sinónimos:
DMSO
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50 ML
MXP 3,749.00
5 X 10 ML
MXP 3,919.00
MXP 3,749.00
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50 ML
MXP 3,749.00
5 X 10 ML
MXP 3,919.00
About This Item
Fórmula lineal:
(CH3)2SO
Número de CAS:
Peso molecular:
78.13
Beilstein/REAXYS Number:
506008
EC Number:
MDL number:
UNSPSC Code:
12191502
PubChem Substance ID:
NACRES:
NA.75
grade:
BioPerformance Certified
technique(s):
cell culture | hybridoma: suitable
cryopreservation: suitable
cryopreservation: suitable
bp:
189 °C (lit.)
vapor pressure:
0.42 mmHg ( 20 °C)
MXP 3,749.00
En stockDetalles
Productos recomendados
grade
BioPerformance Certified
Quality Level
agency
USP XXIV
meets EP testing specifications
meets USP testing specifications
vapor density
2.7 (vs air)
vapor pressure
0.42 mmHg ( 20 °C)
sterility
sterile-filtered
form
liquid
autoignition temp.
573 °F
expl. lim.
42 %, 63 °F
technique(s)
cell culture | hybridoma: suitable
cryopreservation: suitable
impurities
≤0.1% water
color
colorless
refractive index
n20/D 1.479 (lit.)
bp
189 °C (lit.)
mp
16-19 °C (lit.)
solubility
H2O: miscible (completely)
density
1.10 g/mL (lit.)
SMILES string
CS(C)=O
InChI
1S/C2H6OS/c1-4(2)3/h1-2H3
InChI key
IAZDPXIOMUYVGZ-UHFFFAOYSA-N
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General description
El dimetilsulfóxido (DMSO) es un disolvente aprótico. Las moléculas terapéuticas y tóxicas que no son solubles en agua suelen ser solubles en DMSO. El nitrógeno líquido utilizado junto con un crioprotector como el DMSO es un método muy utilizado para conservar las células. El DMSO se ha utilizado para preparar disoluciones madre de varias moléculas y también se utiliza como control para dichas moléculas. Se ha utilizado en el ensayo de migración celular para disolver las células después de la migración. El DMSO probada en BPC también cumple los requisitos de las aplicaciones biotecnológicas y de ingeniería tisular.
Application
El dimetilsulfóxido se ha utilizado como vehículo de control para cultivar e inducir la activación de células estrelladas hepáticas (HSC) in vivo.[1][1] También se ha utilizado para preparar una disolución de reserva para estudiar la citotoxicidad del ácido ferúlico (FA) en las células del núcleo pulposo (NP).[2]
Features and Benefits
Producto esterilizado por filtración listo para usar convenientemente envasado en botellas ámbar con membrana de goma y tapón de rosca. Cumple los requisitos analíticos de la USP y la EP
Caution
Fácilmente superenfriado, vuelve a fundir lentamente a temperatura ambiente. Producto solidificado que puede volver a licuarse por calentamiento a temperatura ambiente sin detrimento del producto.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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Artículos
Overview of good cell banking practices for cell line cryopreservation purposes.
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Hi, what is the difference between DMSO product# D2438 and D2650? What is Hybri-Max vs. BioPerformance certified and meets USP testing specification?
1 answer-
Helpful?
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Purity grade of the product ?
1 answer-
The purity of this product is listed on the lot specific Certificate of Analysis. Please navigate to the DOCUMENTATION section of the Product Detail Page to access a Certificate:
https://www.sigmaaldrich.com/product/sigma/d2438#product-documentationHelpful?
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El DMSO es sensible a la luz, hay que tomar alguna precaución al respecto?
1 answer-
This product is packaged in amber glass and is protected from light. The 10 mL size is packaged in a sealed ampule. Once opened, any content that is not used should also be stored in amber glass. For long term storage, the material may also be stored under inert gas to preserve the shelf life. Note that this material has a tendency to absorb water. Containers should be tightly sealed.
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what are the storing conditions?
1 answer-
This product is stored at room temperature. Store in a well-ventilated place. Keep cool. Please see the Material Safety Data Sheet for more information: https://www.sigmaaldrich.com/sds/sigma/d2438.
Helpful?
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What is the difference between products D2438 and D8418?
1 answer-
The D2438 is a BioPerformance Certified grade product suitable for cell culture, hybridoma creation, and cryopreservation. It is intended for research use only but does meet USP and EP testing requirements. The D8418 is a molecular biology-grade product suitable for DNA sequencing, PCR, and transfection. It has been tested for RNAse and DNAse. The products can be selected based on the application.
Please view the sample Certificate of Analysis for each product to see the testing specifications associated with that item:
https://www.sigmaaldrich.com/certificates/Graphics/COfAInfo/fluka/pdf/PDF734068.pdf
https://www.sigmaaldrich.com/coa/SIGMA/D2438/RNBL8837Helpful?
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What is the basis for the endotoxin specification for Dimethyl sulfoxide (DMSO)?
1 answer-
The industry standard for 'endotoxin free' is less than 1 EU/mL.
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What type of filter is used to sterile-filter Dimethyl sulfoxide (DMSO)?
1 answer-
DMSO can be sterile-filtered through a 0.2 micron PTFE filter. The sterility testing is per the current US Pharmacopoeia method.
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What concentration of DMSO do I use in a cell freezing media?
1 answer-
DMSO is usually used at 1-10%, depending on the cell line. For the cell freezing media that we offer, it is used at 8.7% concentration.
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What is the molarity of DMSO?
1 answer-
DMSO will have a molarity of 14.1M, based on a density of 1.1 g/mL and a molecular weight of 78.13 g/mol.
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Dimethyl sulfoxide (DMSO) should be a liquid. Why did it arrive as a solid?
1 answer-
DMSO supercools easily, and re-melts slowly at room temperature. The melting point is 16-19 °C. The solidified product can be re-liquefied by warming to room temperature without detriment to the product.
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