D0571
Monoclonal Anti-DICER1 antibody produced in mouse
~1.0 mg/mL, clone DCR1, purified immunoglobulin, buffered aqueous solution
Sinónimos:
Anti-DCR1, Anti-HERNA, Anti-Helicase with RNASE motif, Anti-Helicase-MOI, Anti-K12H4.8-LIKE
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200 μL
MXP 11,167.00
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200 μL
MXP 11,167.00
About This Item
Código UNSPSC:
12352203
NACRES:
NA.41
Productos recomendados
origen biológico
mouse
conjugado
unconjugated
forma del anticuerpo
purified immunoglobulin
tipo de anticuerpo
primary antibodies
clon
DCR1, monoclonal
Formulario
buffered aqueous solution
mol peso
antigen ~218 kDa
reactividad de especies
human, mouse, rat
concentración
~1.0 mg/mL
técnicas
western blot: 2.5-5.0 μg/mL using whole extract of human HEK-293T cells
isotipo
IgG1
Nº de acceso UniProt
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... DICER1(23405)
mouse ... Dicer1(192119)
rat ... Dicer1(299284)
Descripción general
Dicer is a member of the RNase III family containing an N-terminal DEXH-box RNA helicase/ATPase domain, followed by a domain of unknown function (DUF283), a PAZ domain, which anchors the 3′-end of the guided siRNA, two RNase III domains, and a dsRBD. The current model for dsRNA cleavage by Dicer predicts that the two ribonuclease III domains of Dicer dimerize to form the catalytic center that is responsible for cleaving long dsRNA in co-operation with two RNA binding domains, PAZ and dsRNA-binding domain.
Monoclonal Anti-DICER1 (mouse IgG1 isotype) is derived from the hybridoma DCR1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a fusion protein corresponding to a fragment of human DICER1.
Aplicación
The antibody may be used in several immunological techniques including immunoblotting and real‐time PCR.
Acciones bioquímicas o fisiológicas
Dicer catalyzes the first step in the RNA interference (RNAi) pathway and initiates formation of the RNA-induced silencing complex (RISC). The dsRNA is processed into small fragments called short interfering RNA (siRNA) or microRNA (miRNA) of typically 21-25 nucleotides long with a two-base overhang on the 3′ end. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. DICER1-deficient colorectal cancer cells have an enhanced ability to initiate tumors and metastasis. Dicer functions as an indirect tumor suppressor, as silencing Dicer expression makes cancer cells more malignant.
Forma física
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Descripción
Precios
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
nwg
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Shuguang Zeng et al.
Oncology reports, 31(2), 867-873 (2013-12-10)
microRNAs (miRNAs) are aberrantly expressed in cancer. An enzyme essential for miRNA processing is Dicer, whose expression is deregulated in diverse types of cancer and correlates with tumor progression. However, whether the regulation of Dicer expression affects tongue squamous cell
Richard I Gregory et al.
Cell, 123(4), 631-640 (2005-11-08)
RNA interference is implemented through the action of the RNA-induced silencing complex (RISC). Although Argonaute2 has been identified as the catalytic center of RISC, the RISC polypeptide composition and assembly using short interfering RNA (siRNA) duplexes has remained elusive. Here
Gunter Meister et al.
Nature, 431(7006), 343-349 (2004-09-17)
Double-stranded RNA (dsRNA) is an important regulator of gene expression in many eukaryotes. It triggers different types of gene silencing that are collectively referred to as RNA silencing or RNA interference. A key step in known silencing pathways is the
Critical role of the miR-200 family in regulating differentiation and proliferation of neurons
Pandey A, et al.
Journal of Neurochemistry, 133(5), 640-652 (2015)
Haruhiko Siomi et al.
Nature, 457(7228), 396-404 (2009-01-23)
The finding that sequence-specific gene silencing occurs in response to the presence of double-stranded RNAs has had an enormous impact on biology, uncovering an unsuspected level of regulation of gene expression. This process, known as RNA interference (RNAi) or RNA
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