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CS1090

Sigma-Aldrich

HMG-CoA Reductase Assay Kit

sufficient for 30 assays (1 mL), sufficient for 100 assays (200 μL)

Sinónimos:

HMGR Assay Kit

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1 KIT
MXP 20,003.00

MXP 20,003.00

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1 KIT
MXP 20,003.00

About This Item

Código UNSPSC:
12161503
NACRES:
NA.28

MXP 20,003.00

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uso

sufficient for 100 assays (200 μL)
 sufficient for 30 assays (1 mL)

Nivel de calidad

200

Condiciones de envío

dry ice

temp. de almacenamiento

−70°C

Información sobre el gen

human ... HMGCR(3156)

Categorías relacionadas

Descripción general

The HMG-CoA Reductase Assay Kit is an important tool for the basic research of cholesterol and other related metabolic pathways.

Aplicación

The HMGR activity kit is designed for the detection of HMG-CoA reductase activity. A major function of the kit is to screen for various inhibitors and activators of the purified catalytic subunit of the enzyme, HMG-CoA reductase, which may play a crucial role in therapeutics. The assay is based on the spectrophotometric measurement of the decrease in absorbance at 340 nm, which represents the oxidation of NADPH by the catalytic subunit of HMGR in the presence of the substrate HMG-CoA.

Acciones bioquímicas o fisiológicas

HMGR (3-hydroxy-3-methylglutaryl-CoA reductase) is a transmembrane glycoprotein located on the endoplasmic reticulum.[1] This enzyme catalyzes the four-electron reduction of HMG-CoA to coenzyme A (CoA) and mevalonate, which is the rate-limiting step in sterol biosynthesis.[2] The activity of HMGR is controlled through synthesis, degradation, and phosphorylation in order to maintain the concentration of mevalonate derived products. In addition to the physiological regulation of HMGR, the human enzyme has been targeted successfully by drugs in the clinical treatment of high serum cholesterol levels.[3][4] Controlling serum cholesterol levels has an important therapeutic role since hypercholesterolemia often leads to the development of atherosclerosis and subsequently to cardiovascular pathologies, which might result in myocardial infarction and stroke. It has been suggested that a disturbance of cholesterol homeostasis contributes to the development of a chronic inflammatory state.[5]

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS
  • N6505β-Nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate, ≥95% (HPLC) 25 mgSDS

Código de clase de almacenamiento

11 - Combustible Solids

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Certificados de análisis (COA)

Lot/Batch Number
0000415146
0000415146
0000364958
0000364958
0000261885

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Rosana Aparecida Manólio Soares et al.
International journal of molecular sciences, 16(2), 4150-4160 (2015-02-19)
The objective of this study was to identify the major peptides generated by the in vitro hydrolysis of Amaranthus cruentus protein and to verify the effect of these peptides on the activity of 3-hydroxy-3-methyl-glutaryl-CoA reductase (HMG-CoA reductase), a key enzyme
Mohammad Azizur Rahman et al.
BioMed research international, 2014, 828149-828149 (2014-06-25)
Oxidation of low-density lipoprotein (LDL) has been strongly suggested as the key factor in the pathogenesis of atherosclerosis. Mushrooms have been implicated in having preventive effects against chronic diseases due especially to their antioxidant properties. In this study, in vitro
Yosie Andriani et al.
Journal of advanced pharmaceutical technology & research, 11(1), 30-35 (2020-03-11)
Atherosclerosis is a leading cause of death worldwide. The adverse side effects of currently available drugs urge to find more effective and safe remedial agents. Alternative candidates from natural resources are of great consequence in the emerging of new drugs.
R Kleemann et al.
Current drug targets. Cardiovascular & haematological disorders, 5(6), 441-453 (2006-03-01)
Besides classical risk factors such as hypercholesterolemia and hypertension, chronic subacute inflammation has recently been recognized as an important force driving the development of atherosclerosis, the most common underlying cause of myocardial infarction and stroke. There is compelling evidence that
G A Holdgate et al.
Biochemical Society transactions, 31(Pt 3), 528-531 (2003-05-30)
The statins are inhibitors of 3-hydroxy-3-methylglutaryl CoA (HMG-CoA) reductase (HMG-CoAR), and are utilized to decrease levels of atherogenic lipoproteins in patients with, or who are at high risk of, cardiovascular disease. This study describes the inhibition of a recombinant, catalytic
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HMG-CoA Reductase (HMGR) Assay Kit

3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) is a transmembrane glycoprotein, located on the endoplasmic reticulum.

Questions

1–10 of 10 Questions  

  1. How many samples can we analyse using 1Kt?

    1 answer

    1. Tables 1 and 2 provide specific instructions for sample preparation for reaction volumes in a cuvette (1 mL) or a 96 well plate. The limiting reagent based upon the supplied kit components is the HMG-COA. There is 2 mL of HMG-COA supplied and 0.18 mL used in total for the blank, activity and inhibitor well preparations. This means that approximately 33 wells or 30 assays can be performed as indicated in the kit bulletin.

      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/240/783/cs1090pis-ms.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  3. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  4. What is the concentration of the HMG CoA substrate (S7447)?

    1 answer

    1. The concentration of the HMG CoA substrate, product number S7447, is 0.5% in water.

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  5. Is this suitable for the analysis of compounds dissolved in DMSO for comparing the ability of some compounds to inhibit HMGCR? Specifically, using 1 ul of the compound solution (0.05, 0.5, 5 mM stock buffer) and 1 ul DMSO (solvent alone) as control, the signal @ 340 nM did not significantly decrease after a 10-minute kinetic program (signal capture every 20 seconds according to the protocol). There was only a 15-20 percent decrease in the read. Is this normal, or is DMSO incompatible with the enzyme reaction, or can some improvement be made?

    1 answer

    1. As per the QC specialist group, in the product CS1090, there isn't any experience with DMSO (all kit's components are without DMSO), so the information cannot provided about DMSO incompatibility. After reviewing the raw data, a good slope was observed with the enzyme; for example, with 6ug (10ul) enzyme, the slope was 0.3-0.5.

      Helpful?

  6. •What is the concentration of the HMG-CoA (Cat.No. S7447)? and What solvent is it dissolved in?

    1 answer

    1. The concentration of HMG-CoA is the composition of 99.5 % water and 0.5 % (DL-3-Hydroxy-3-methylglutaryl) coenzyme aso-salt trihydrate.

      Helpful?

  7. Product CS1090, kit component of HMG-CoA Reductase Assay Kit Product I5909, Pravastatin, is the concentration 100uM correct?

    1 answer

    1. Yes, the concentration for Pravastatin in this kit is 100 micromolar (100 μM)

      Helpful?

  8. What is the lowest concentration that you can use Pravastatin and observe inhibition in Product CS1090, HMG-CoA Reductase Assay kit?

    1 answer

    1. For Product CS1090, HMG-CoA Reductase Assay kit we do not observe any inhibition when using Pravastatin at 25 or 50 nM.  We observe inhibition of HMG-CoA Reductase with 250 nM pravastatin.

      Helpful?

  9. For Product CS1090, HMG-CoA Reductase Assay Kit, what is the concentration of kit component Product I5909, Pravastatin?

    1 answer

    1. The concentration of Pravastatin, Product I5909 in Product CS1090, HMG-CoA Reductase Assay Kit,  is 100 uM solution in assay buffer.

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  10. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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