LB253

98062330, human blood, Lymphoblastoid

• UNSPSC Code: 41106514

Propiedades

• product name: LB253, 98062330
• biological source: human blood
• growth mode: Suspension
• karyotype: Not specified
• morphology: Lymphoblastoid
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Human lymphocytes, Xeroderma pigmentosum group C, EBV-transformed

Cell Line Description

Derived from mononuclear cells from the peripheral blood of a 67 year old male. The patient was diagnosed with Xeroderma pigmentosum group C and showed spontaneous regression of melanomas. A normal level of natural killer (NK) cell activity and number, despite marked lymphopenia, was reported. The original sample was used in UV irradiation studies where UV-sensitivity of lymphocytes and fibroblasts was detected. In addition, a defect in excision repair was reported.

application

DNA repair deficiency studies, hprt mutant analysis

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11,12
D16S539: 9,13
D5S818: 11,12
D7S820: 8,12
THO1: 6,9.3
TPOX: 8
vWA: 17,19

Culture Medium

RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).

Subculture Routine

Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml 5%

Other Notes

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