B1D6

98042014, Reactivity: Anti-Annexin II, human

• UNSPSC Code: 41106514

Propiedades

• biological source: Mouse x Mouse Hybridoma
• ImmunoDon: BALB/c mouse spleen
• Myeloma: P3X63Ag8
• Reactivity: Anti-annexin II, human
• technique(s): cell culture | mammalian: suitable
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Not specified

Cell Line Description

The hybridoma B1D6 was obtained by immunising BALB/c mice with human placental extract followed by intravenous injections with purified Fc gamma-receptor (receptor for the Fc portion of immunoglobulins, FcR). Spleen cells were then fused with the myeloma P3X63Ag8 to establish B1D6 secreting IgG1, kappa, monoclonal antibodies. This antibody has been shown to stain FcR-positive areas in sections of placental tissues, inhibited the haemadsorption of placental tissue of erythrocytes sensitised with IgG antibodies and the agglutination of these erythrocytes by FcR.

Immunogen

Human placenta FcR

Application

FcR studies

Culture Medium

RPMI 1640 + 2 mM glutamine + 10% FBS

Subculture Routine

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO₂; 37 °C

Other Notes

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