HR5

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Código UNSPSC: 41106514

Propiedades

• origen biológico: human cervix
• envase: tube of 5 μg 95091409-DNA-5UG; pkg of vial of cells 95091409-1VL
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Epithelial-like
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human cervix carcinoma, HeLa derivative, transformed

Descripción línea celular

HR5 cells were obtained after transfection of HeLa cells with plasmids coding for a reverse tetracycline-controlled transactivator encoding a nuclear localisation sequence (rt TA-nls) and transferring G418 resistance. This transactivator (a fusion between the Tet repressor and the activating domain of the herpes simplex virus VP16 transcription activation domain) can stimulate the expression of a reporter gene up to 10E5-fold. HR5 is the parent cell line for HR5-CL11 (Sigma Catalogue number.95091411).

Aplicación

Gene expression and activation studies

Medio de cultivo

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 2x1,000 to 2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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