13C4

Reactivity: Anti-Shigella-like Toxin I (SLTI), 95032701

• UNSPSC Code: 41106514

Propiedades

• biological source: Mouse x Mouse Hybridoma
• ImmunoDon: BALB/c mouse spleen
• Myeloma: Sp2/0 Ag-14
• Reactivity: Anti-Shigella-like Toxin I (SLTI)
• technique(s): cell culture | mammalian: suitable
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Mouse Sp2/0 Ag-14 x Mouse BALB/c spleen

Cell Line Description

The antibody is directed against Shigella toxin and neutralises Shigella toxin activities from Shigella dysenteriae Type 1 and S. flexneri. It also reacts with the ß subunit of SLTI and neutralises the Shigella-like activities of E.coli, Vibrio cholerae, V.parahaemolyticus and Salmonella typhimurium.

Immunogen

Purified Shigella-like toxin from E.coli H30

Application

Study of shiga toxins.

Culture Medium

DMEM + 2mM glutamine + 0.15mg/ml oxaloacetate + 0.2U/ml Bovine Insulin + 0.05mg Sodium Pyruvate (NaP) + 5-10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

Other Notes

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