KB (HeLa derivative)

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 94050408, human cervix, Epithelial-like

• Código UNSPSC: 41106514

Propiedades

• origen biológico: human cervix
• envase: tube of 5 μg 94050408-DNA-5UG; pkg of vial of cells 94050408-1VL
• modo de crecimiento: Adherent
• cariotipo: 2n = 46
• morfología: Epithelial-like
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human cervix carcinoma

Descripción línea celular

Originally derived from an epidermoid carcinoma of the mouth of an adult male Caucasian. It was one of the early attempts to isolate and serially propagate a human cell line directly on glass as a monolayer. NB: This cell line contains HeLa marker chromosomes and expresses type A G6PD. For further information see Nature 1976;259:211; In Vitro 1978;14:469. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.

Aplicación

KB has been used extensively in studies of cell nutrition and metabolism, cancer chemotherapy screening, tumourigenicity and viruses e.g. susceptible to Polio virus type I and Adenovirus type 3.

Medio de cultivo

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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