NCTC 2071

90050803, mouse connective tissue, Fibroblast

• UNSPSC Code: 41106514

Propiedades

• product name: NCTC 2071, 90050803
• biological source: mouse connective tissue
• growth mode: Adherent
• karyotype: Modal no. 63
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Mouse C3H/An connective tissue

Cell Line Description

Derivative of NCTC clone 929 and was established in chemically defined medium (NCTC 109). It was subsequently adapted to cysteine-free medium (NCTC 135). The cells have been used in nutritional, immunological and toxicological studies; nutritional, metabolic and enzyme studies; preservation and storage of viable tissue; fluid suspension culture methods and replicate culture techniques and quantitative cell culture methods.

Application

Nutritional, immunological and toxicological studies

Culture Medium

NCTC 135 100% + 2mM Glutamine.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:5 i.e. seeding at 5x10,000 to 2x100,000 cells/cm² by scraping off the cells and resuspending into new flasks; 5% CO₂; 37°C. Freeze in 9% glycerol/91% FBS.

Other Notes

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