4-11

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Sinónimos: HeLa
• Código UNSPSC: 41106514

Propiedades

• origen biológico: human cervix
• envase: tube of 5 μg 11033103-DNA-5UG; pkg of vial of cells 11033103-1VL
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Epithelial
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice

Descripción

Origen línea celular

HeLa, genetic modification, human androgen receptor, firefly luciferase reporter gene, MMTV promoter

Descripción línea celular

4-11 is a HeLa cell line stably transfected with two constructs: the human androgen receptor (hAR) expression construct; a firefly luciferase reporter gene under the control of the MMTV promoter. 4-11 can be used to detect androgenic activity of chemicals.

Perfil del ADN

STR-PCR Data:

Amelogenin: X
CSF1PO: 9,10
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18

Medio de cultivo

DMEM (D6546) + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442) + 16 μg/ml Blasticidin S HCL + 800 μg/ml G418.

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:4 to 1:10, i.e. seeding at 2 x 10⁴ cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.

Otras notas

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Información legal

US7537904B1 N Ooe & Matsunaga H 2009.Cell for measuring the ability to control the activity of a ligand-responsive transcription control factor.

Cláusula de descargo de responsabilidad

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.