utr-1

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Código UNSPSC: 41106514

Propiedades

• origen biológico: Human x Mouse Hybridoma
• envase: tube of 5 μg 10080904-DNA-5UG; pkg of vial of cells 10080904-1VL
• ImmunoDon: Mouse (Mus musculus) spleen
• Mieloma: PAI
• Reactividad: Human CD120b (TNF receptor p75)
• técnicas: cell culture | mammalian: suitable
• isotipo: IgG1
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Aplicación

Sandwich ELISA for soluble p75 TNF-R. No competition with TNF.

Medio de cultivo

Iscoves Modified Dulbeccos Medium (IMDM) + 2 mM glutamine + 25 U/ml Interleukin-6 (IL-6) + 5-10% Fetal Bovine Serum (FBS). Where the cells are grown under static conditions, 0.02% EDTA may be required to dislodge them from the plastic.

Rutina de subcultivo

Seed cultures between 1-3 x 10⁵ cells/ml; Saturation density 8-10 x 10⁵ 5% CO₂; 37 °C

Otras notas

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