UM-UC-13

8090508, human bladder, Epithelial

• Sinónimos: Human UM-UC-13, UM-UC-13 Cell Line
• UNSPSC Code: 41106514

Propiedades

• product name: UM-UC-13, 08090508
• biological source: human bladder
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): metastasis
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Human transitional cell carcinoma lymphatic metastasis

Cell Line Description

UM-UC-13 was produced from a lymphatic metastasis from a transitional cell carcinoma of the bladder. UM-UC-13 produced tumours in female athymic nude mice after 36 days. Susceptibility to adenoviral mediated gene transduction and expression of p53, p16, retinoblastoma protein and coxsackie adenovirus receptor were studied.

DNA Profile

STR-PCR Data:

Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 8,12
D16S539: 11
D5S818: 9,13
D7S820: 8,9
THO1: 9
TPOX: 8
vWA: 16,18

Culture Medium

EMEM (EBSS) + 2 mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 1-2x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37 °C.

Other Notes

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Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.