HeLa (AC-free), NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., 08011102
origen biológico
human cervix
envase
tube of 5 μg 08011102-DNA-5UG pkg of vial of cells 08011102-1VL
Human cervical carcinoma, serum-free, animal component (AC) free
Descripción línea celular
HeLa cell line (Sigma catalogue no. 93021013) adapted to grow in animal component free medium (EX-CELL HeLa Serum-Free Medium for HeLa Cells Sigma cat no 14591C).
Aplicación
Virus studies
Medio de cultivo
EX-CELL HeLa Serum-Free Medium (Sigma cat no 14591C)+ 2mM Glutamine. When freezing cells down use 50:50 fresh culture medium:conditioned medium plus 10% DMSO.
Rutina de subcultivo
Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Tran
Otras notas
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Certificados de análisis (COA)
Lot/Batch Number
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