C17.2

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 7062902, mouse brain, Neuronal

• Código UNSPSC: 41106514

Propiedades

• origen biológico: mouse brain
• envase: tube of 5 μg 07062902-DNA-5UG; pkg of vial of cells 07062902-1VL
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Neuronal
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse multipotent neural progenitor or stem-like cells

Descripción línea celular

An immortalised mouse neural progenitor cell line capable of differentiation in vitro. The cell line was established by retorviral-mediated transduction of the avian myc oncogene into mitotic progenitor cells of neonatal mouse cerrebellum. Mouse strain CD1 x C57BL/6. This cell line is a valuable tool for in vitro and in vivo studies aimed at understanding the control of cell fate and differentiation of neural progenitors. The MMLV retrovirus vector used for the immortalisation process contained a neo resistance gene transcribed from an internal SV40 promoter. Therefore the cells are neo resistant. The morphology of the cells may change over time. Cells plated at low density may tend to become more process bearing whereas those plated more densely may tend to become flat and non-process bearing.

Medio de cultivo

DMEM + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS). Flasks should be pre-coated with poly-L-lysine at 10 μg/ml in sterile distilled water.

Rutina de subcultivo

Feed cells weekly with 50% conditioned medium and 50% fresh medium or split 1:10-1:20 in fresh medium using trypsin/EDTA. 5% CO₂; 37 °C. Split sub-confluent cultures 1:10 - 1:20, i.e., seeding at 2-4 x 10,000 cells/cm². Flasks should be pre-coated with poly-L-lysine at 10 μg/ml in sterile distilled water. Cells can be split as dilute as 1:50, but the phenotype may change, i.e., cells may appear flat with an epithelial-like morphology which usually do not stain for neurofilament or GFAP.

Otras notas

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