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C6854

Sigma-Aldrich

Cathepsin L from human liver

≥0.5 units/mg protein, solution

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25 μG
MXP 11,123.00

MXP 11,123.00

Disponible para envío el08 de abril de 2025Detalles

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25 μG
MXP 11,123.00

About This Item

Número de CAS:
60616-82-2
Comisión internacional de enzimas:
3.4.22.15 (BRENDA, IUBMB)
Número MDL:
MFCD00163235
Código UNSPSC:
12352200
NACRES:
NA.32

MXP 11,123.00

Disponible para envío el08 de abril de 2025Detalles

Solicitar un pedido a granel

origen biológico

human liver

Nivel de calidad

200

Formulario

solution

actividad específica

≥0.5 units/mg protein

técnicas

activity assay: suitable

intervalo de pH

4.5—5.5

Nº de acceso UniProt

P07711

aplicaciones

pharmaceutical

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

Información sobre el gen

human ... CTSL1(1514)

Descripción general

The cathepsin L (CTSL) gene is mapped to human chromosome 9q21.33. It encodes a lysosomal cysteine proteinase that belongs to the peptidase C1 family. The enzyme is a dimer of a heavy (175 amino acids) and a light chain (42 amino acids) linked by disulfide bonds.

Aplicación

Cathepsin L (CTSL) from human liver has been used:
  • to digest mutant transforming growth factor β-induced (TGFBIp) and LC3 (autophagosomes marker) proteins in vitro
  • to digest the pH-dependent fibril of a pre-melanosomal protein (Pmel17) repeat domain (RPT) isoform and study the effect of pH on sRPT aggregation kinetics by tryptophan fluorescence
  • to analyze the inhibition of CTSL by small molecules or drugs in a cell-free system

Acciones bioquímicas o fisiológicas

Cathepsin L (CTSL) exhibits endopeptidase activity and breaks peptide bonds with aromatic residues. This enzyme is functional at pH 3.0–6.5 with thiol compounds. Elastin, collagen, and α-1 protease inhibitors act as substrates for cathepsin L (CTSL). CTSL cleaves several proteins including enzymes, receptors, and transcription factors. It is involved in antigenic peptide production and controls B-cells homeostasis. It is associated with myofibril necrosis and tumor progression. Increased expression of CTSL promotes metastasis and relates to poor prognosis in cancer patients. Increased expression of CTSL is observed in breast cancer. CTSL is implicated in glioblastoma multiforme (GBM), middle east respiratory syndrome (MERS), gingival overgrowth, and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). It has a higher specific activity than cathepsin B and H in the degradation of a variety of physiological protein substrates.

Definición de unidad

One unit will hydrolyze 1.0 μmole of Z-Phe-Arg-AFC per minute at pH 5.5 at 25 °C.

Forma física

Solution in in 20 mM malonate, pH 5.5, 1 mM EDTA, and 400 mM NaCl.

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Referencia del producto
Descripción
Precios

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000266834
0000266834
0000264664
0000264664
SLCR2105

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Seung-Il Choi et al.
Journal of cellular and molecular medicine, 24(18), 10343-10355 (2020-07-16)
Granular corneal dystrophy type 2 (GCD2) is the most common form of transforming growth factor β-induced (TGFBI) gene-linked corneal dystrophy and is pathologically characterized by the corneal deposition of mutant-TGFBIp. The defective autophagic degradation of pathogenic mutant-TGFBIp has been shown
Stefan Tholen et al.
Cellular and molecular life sciences : CMLS, 71(5), 899-916 (2013-07-03)
Endolysosomal cysteine cathepsins functionally cooperate. Cathepsin B (Ctsb) and L (Ctsl) double-knockout mice die 4 weeks after birth accompanied by (autophago-) lysosomal accumulations within neurons. Such accumulations are also observed in mouse embryonic fibroblasts (MEFs) deficient for Ctsb and Ctsl.
Bing-Jie Lv et al.
Atherosclerosis, 230(1), 100-105 (2013-08-21)
Cathepsin L (CatL), cathepsin K (CatK), and cathepsin V (CatV) are potent elastases implicated in human arterial wall remodeling. Whether plasma levels of these cathepsins are altered in patients with abdominal aortic aneurysms (AAAs) remains unknown. Plasma samples were collected
Gustavo E Chavarria et al.
European journal of medicinal chemistry, 58, 568-572 (2012-11-22)
Kinetic analysis of the mode of inhibition of cathepsin L by KGP94, a lead compound from a privileged library of functionalized benzophenone thiosemicarbazone derivatives, demonstrated that it is a time-dependent, reversible, and competitive inhibitor of the enzyme. These results are
M Kathryn Liszewski et al.
Immunity, 39(6), 1143-1157 (2013-12-10)
Complement is viewed as a critical serum-operative component of innate immunity, with processing of its key component, C3, into activation fragments C3a and C3b confined to the extracellular space. We report here that C3 activation also occurred intracellularly. We found
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Questions

  1. Hello, What is the protocol to bring this up in solution to have a concentration of 0.5 units/µL? Does units mean µg where the amount needed for performing digestions? Is there a protocol associated with this?

    1 answer

    1. This product is supplied as a solution. The minimum activity for each lot will be ≥0.5 units/mg protein. The specific activity of each lot is reported on the Certificate of Analysis. See the link below to review a sample Certificate:
      https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/C6854-25UGSLCR2105.pdf

      The product is supplied in 20 mM malonate buffer, pH 5.5, with 1 mM EDTA and 400 mM NaCl. One unit is defined as the amount of Cathepsin L that hydrolyzes 1 uM Z-PHE-Arg-AFC per minute at 22 - 25 °C, in 400 mM Sodium Acetate, pH 5.5, containing 4 mM EDTA and 8 mM DTT.

      Please access the product datasheet at the link below:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/130/380/c6854dat.pdf

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