The Hematopoietic Progenitor Cell (HPC) Expansion Medium DXF has been designed for the maximum expansion of primitive HPCs in a fully defined, xeno-free culture environment. Due to the utilization of exclusively synthetic, recombinant or plant-sourced materials, this medium is defined and free of all animal-derived components and substances of human origin with Human Serum Albumin as the only exception.Recommended for:Human Mononuclear Cells (hMNC)Human CD34+ Progenitor Cells (hCD34+-CB)Human Hematopoietic Progenitor Cells (HPCs)
Quality
All lots of PromoCell Hematopoietic Progenitor Media are subjected to comprehensive quality control tests using primary human mononuclear cells as well as purified hematopoietic progenitor cells from cord blood. Each lot of PromoCell HPC Expansion Medium DXF is tested for the ability to support the expansion of hematopoietic progenitor cells with a primitive phenotype. Each lot of PromoCell Hematopoietic Progenitor Medium is tested for the capacity to induce directed differentiation into hematopoietic lineages using purified CD34+ progenitor cells from cord blood. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).
Preparation Note
The Medium consists of a bottle of Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium results in the complete Medium. In order to achieve optimal performance, the complete Medium must be supplemented with additional growth factors by the user, e.g. the optionally available Cytokine Mix E (C-39890, 1 ml or C-39891, 5 ml) containing recombinant human TPO, SCF, flt-3 ligand and IL-3. Alternatively, supplement the HPC Expansion Medium DXF with growth factors of your choice.
Storage and Stability
Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix or Cytokine Mix at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Medium supplemented with Cytokine Mix E should be used within 2 weeks after addition of the cytokines. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.
CD34+ Progenitor Cells are suitable for diverse studies, as well as for the directed differentiation into more committed blood cell phenotypes and endothelial lineages.
Hematopoietic stem cell culture protocols for isolation, expansion, and differentiation of CD34+ cells including CFU assays.
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