C2014

Colagenasa from Clostridium histolyticum

Name: Colagenasa from <I>Clostridium histolyticum</I>
Brand: SIGMA
Price: 2786 MXP

0.2 μm filtered, Type V-S, ≥1 FALGPA units/mg solid, ≥125 CDU/mg solid

Sinónimos:

Clostridiopeptidasa A

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50 MG

MXP 2,786.00

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Número CAS:

9001-12-1

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-582-9

MDL number:

MFCD00130830

Número CE:

3.4.24.3 (BRENDA, IUBMB)

Specific activity:

≥1 FALGPA units/mg solid, ≥125 CDU/mg solid

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sterility

0.2 μm filtered

form

lyophilized powder

specific activity

≥1 FALGPA units/mg solid, ≥125 CDU/mg solid

mol wt

68-125 kDa

storage temp.

−20°C

Quality Level

200

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Este artículo	C5894	C9263	C7657
Sigma-Aldrich C2014 Collagenase from Clostridium histolyticum	Sigma-Aldrich C5894 Collagenase from Clostridium histolyticum	Sigma-Aldrich C9263 Collagenase from Clostridium histolyticum	Sigma-Aldrich C7657 Collagenase from Clostridium histolyticum
specific activity ≥1 FALGPA units/mg solid, ≥125 CDU/mg solid	specific activity ≥125 CDU/mg solid, 0.5-5.0 FALGPA units/mg solid	specific activity ≥1 FALGPA units/mg solid, >125 CDU/mg solid	specific activity ≥800 CDU/mg solid, 2-5 FALGPA units/mg solid
form lyophilized powder	form lyophilized powder	form powder	form powder
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
mol wt 68-125 kDa	mol wt 68-130 kDa	mol wt 113-114 kDa	mol wt 68-130 kDa
sterility 0.2 μm filtered	sterility non-sterile; 0.2 μm filtered	sterility -	sterility -
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200

Application

Collagenase has been used in the preparation of arterial tissue for the study of Advanced Glycosylation End Products (AGE). The enzyme has also been used along with other proteases for the disaggregation of human tumor, mouse kidney, human brain, lung epithelium and many other tissues. It is also effective in liver and kidney perfusion studies, digestion of pancreas, and isolation of nonparenchymal hepatocytes.

Biochem/physiol Actions

La colagenasa es activada por cuatro átomo-gramos de calcio por mol de enzima. Es inhibida por el ácido etilenglicol-bis(beta-aminoetil éter) - N, N, N′,N′-tetraacético, el beta-mercaptoetanol, el glutatión, el ácido tioglicólico y la 8-hidroxiquinolina.

Effective release of cells from tissue requires the action of both collagenase enzymes and the neutral protease. Collagenase is activated by four gram atom calcium (Ca²⁺) per mole enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa (pH optimum: 6.3-8.8). The enzyme recognizes the sequence -R-Pro-8-X-Gly-Pro-R-, where X is most often a neutral amino acid.

Preparation Note

Prepared from Type V (C9263)

Analysis Note

Also contains clostripain, nonspecific neutral protease, and tryptic activities.

Other Notes

Una unidad de digestión de colágeno (CDU) libera péptidos del colágeno del tendón de Aquiles bovino que equivalen en la reacción de color de la ninhidrina a 1,0 μmoles de leucina en 5 horas a pH 7,4 y 37 °C en presencia de iones calcio. Una unidad de hidrólisis FALGPA hidroliza 1,0 μmoles de furilacriloil-Leu-Gly-Pro-Ala por minuto a 25°C. Una unidad de proteasa neutra hidroliza la caseína para producir color equivalente a 1,0 μmoles de tirosina por 5 horas a pH 7,5 y 37°C. Una unidad de clostripaína hidroliza 1,0 μmoles de BAEE por minuto a pH 7,6 y 25°C en presencia de DTT.

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Referencia del producto

Descripción

Precios

F5135

N-[3-(2-Furyl)acryloyl]-Leu-Gly-Pro-Ala

pictograms

GHS08,GHS07

signalword

Danger

hcodes

H315,H319,H334,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificados de análisis (COA)

Lot/Batch Number

0000503750

0000302834

0000137861

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Multiple concurrent collagenase clostridium histolyticum injections to Dupuytren's cords: an exploratory study.

Stephen Coleman et al.

BMC musculoskeletal disorders, 13, 61-61 (2012-05-01)

Dupuytren's contracture (DC) is a progressive fibroproliferative disorder characterized by development of nodules and collagen cords within the palmar fascia of the hand. Collagenase clostridium histolyticum (CCH) is currently approved in adults with DC for the nonsurgical treatment of a

Collagenase clostridium histolyticum injection for the treatment of Dupuytren's contracture.

F T D Kaplan

Drugs of today (Barcelona, Spain : 1998), 47(9), 653-667 (2011-10-06)

Collagenase clostridium histolyticum is a novel treatment for Dupuytren's contracture, approved by the U.S. Food and Drug Administration in February 2010. Prior to its availability, surgery was the only treatment for contracture related to this disorder. Dupuytren's disease is a

Phase 2b study of the clinical efficacy and safety of collagenase Clostridium histolyticum in patients with Peyronie disease.

Martin Gelbard et al.

The Journal of urology, 187(6), 2268-2274 (2012-04-17)

Collagenase Clostridium histolyticum is an investigational nonsurgical treatment for Peyronie disease. In this phase 2b, double-blind, randomized, placebo controlled study we determined the safety and efficacy of collagenase C. histolyticum and assessed a patient reported outcome questionnaire. A total of

In vitro study of novel collagenase (XIAFLEX®) on Dupuytren's disease fibroblasts displays unique drug related properties.

Farhatullah Syed et al.

PloS one, 7(2), e31430-e31430 (2012-03-03)

Dupuytren's disease (DD) is a benign, fibroproliferative disease of the palmar fascia, with excessive extracellular matrix (ECM) deposition and over-production of cytokines and growth factors, resulting in digital fixed flexion contractures limiting hand function and patient quality of life. Surgical

▾Xiapex for Dupuytren's contracture.

Drug and therapeutics bulletin, 49(12), 138-141 (2011-12-16)

Dupuytren's contracture is a benign, progressive disabling condition of the hands. Traditionally, surgery (e.g. fasciotomy, fasciectomy, joint fusion and amputation) has been the only effective treatment but it is not an option for every patient. A potential alternative intervention is

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Protocolos

Enzymatic Assay of Collagenase (EC 3.4.24.3) using FALGPA (N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala)

To measure collagenase activity, N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala is used in a continuous spectrophotometric rate determination at 345 nm. Collagenase hydrolyzes collagen peptide bonds.

Contenido relacionado

Product Information Sheet - C2014

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