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B5282

Sigma-Aldrich

Bold Modified Basal Freshwater Nutrient Solution

50 ×, liquid, suitable for plant cell culture

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500 ML
MXP 1,467.00

MXP 1,467.00

Fecha estimada de envío17 de abril de 2025

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500 ML
MXP 1,467.00

About This Item

UNSPSC Code:
10171502
NACRES:
NA.72

MXP 1,467.00

Fecha estimada de envío17 de abril de 2025

Solicitar un pedido a granel

sterility

sterile-filtered

Quality Level

400

form

liquid

concentration

50 ×

technique(s)

cell culture | plant: suitable

application(s)

agriculture

Storage temp.

2-8°C

Categorías relacionadas

Application

Bold Modified Basal Freshwater Nutrient Solution, 50x is a chemstock used to prepare Bold Modified Freshwater Nutrient Solution for use in phycology/algae studies.

Formula variant

With major salts and trace elements. Modified with trace elements as requested for ASTM TOXICITY TESTS E1193 and E1295 conducted with Daphnia species.

Reconstitution

Use at 20ml/L

Phycology Media composition and preparation from powder

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
RNBL8217
RNBL1419
RNBK5851
RNBJ3857
RNBJ2330

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Protocolos

Phycology Media in Plant Tissue Culture

Phycology media can be prepared from concentrated solutions or from powdered salt mixtures. The concentrated solutions are complete, including vitamins, and should be stored frozen.

Questions

1–2 of 2 Questions  

  1. Can this medium be combined with agar to make a solid medium?

    1 answer

    1. While not tested as such, a gelling agent may be added to this solution to form a solid medium. As this is a prepared solution, the agar would be added directly to the medium.

      Please see the link below, step 7, to review the general phycology media preparation protocol: https://www.sigmaaldrich.com/US/en/technical-documents/protocol/cell-culture-and-cell-culture-analysis/plant-tissue-culture/phycology-media

      Helpful?

  2. Is this suitable for freshwater diatom culture?

    1 answer

    1. This product is tested for culture using green algae that are not specifically designated as diatomaceous. This application may need to be validated by the end user. The formulation is prepared according to the the reference below:
      Nichols, H. W. Growth Media-Freshwater in: Stein, J.r. (ed) Phycological Methods, Cambridge Univ. Press, 7-23, 1973.

      Helpful?

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