B3795
Brain-derived neurotrophic factor human
≥95% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Sinónimos:
Abrineurin, BDNF
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Nombre del producto
Brain-derived neurotrophic factor human, BDNF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
origen biológico
human
Nivel de calidad
recombinante
expressed in E. coli
Ensayo
≥95% (SDS-PAGE)
Formulario
lyophilized powder
calidad
endotoxin tested
mol peso
~27 kDa
envase
pkg of 10 μg
pkg of 5 μg
condiciones de almacenamiento
avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)
técnicas
cell culture | mammalian: suitable
impurezas
≤1.00 EU/μg Endotoxin level
Nº de acceso UniProt
temp. de almacenamiento
−20°C
Descripción general
BDNF is a member of the neurotrophin family of growth factors that includes NGF, NT-3, and NT-4. All neurotrophins have six conserved cysteine residues and share a 55% sequence identity at the amino acid level. BDNF has been shown to enhance the survival and differentiation of several classes of neurons in vitro, including neural crest and placode-derived sensory neurons, dopaminergic neurons in the substantia nigra, basal forebrain cholinergic neurons, hippocampal neurons, and retinal ganglial cells.1 BDNF is expressed within peripheral ganglia and is not restricted to neuronal target fields, raising the possibility that BDNF has paracrine or even autocrine actions on neurons as well as non-neuronal cells.
The active form of BDNF recombinant human protein (27 kDa) is a dimer formed by two identical 119 amino acid subunits held together by strong hydrophobic interactions
The active form of BDNF recombinant human protein (27 kDa) is a dimer formed by two identical 119 amino acid subunits held together by strong hydrophobic interactions
Aplicación
Acciones bioquímicas o fisiológicas
Brain-derived neurotrophic factor (BDNF) is a 13.6 kDa (or 27.2 kDa dimer) member of the neurotrophin family. BDNF has identical amino acid sequence in human, mouse, and pig with full cross-reactivities. BDNF is important in development and maintenance of neuronal populations within the central nervous system or cells directly associated with it. BDNF has been shown to enhance the survival and differentiation of several classes of neurons in vitro, including neural crest and placode-derived sensory neurons, dopaminergic neurons in the substantia nigra, basal forebrain cholinergic neurons, hippocampal neurons, and retinal ganglial cells.
Forma física
lyophilized from a sterile 0.2 μm filtered solution with BSA
Nota de análisis
The biological activity is determined by its ability to stimulate the proliferation of human neuroblastoma cells, SH-SY5Y.
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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Protocolos
Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.
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