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A9655

Sigma-Aldrich

Auramine O

Dye content, ≥80%, certified by the Biological Stain Commission, powder

Sinónimos:

4,4′-(Imidocarbonyl)bis(N,N-dimethylaniline) monohydrochloride, Basic Yellow 2, Pyoctaninum aureum

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About This Item

Fórmula empírica (notación de Hill):
C17H21N3 · HCl
Número de CAS:
2465-27-2
Peso molecular:
303.83
Colour Index Number:
41000
Beilstein/REAXYS Number:
4030061
EC Number:
219-567-2
MDL number:
MFCD00012484
UNSPSC Code:
12171500
PubChem Substance ID:
24891484
NACRES:
NA.47

product name

Auramine O, Dye content ≥80 %, certified by the Biological Stain Commission

grade

certified by the Biological Stain Commission

form

powder

composition

Dye content, ≥80%

color

yellow to green

mp

>250 °C (dec.) (lit.)

solubility

ethanol: 1 mg/mL, yellow to orange

λmax

370 nm in H2O
432 nm in H2O

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Cl[H].CN(C)c1ccc(cc1)C(=N)c2ccc(cc2)N(C)C

InChI

1S/C17H21N3.ClH/c1-19(2)15-9-5-13(6-10-15)17(18)14-7-11-16(12-8-14)20(3)4;/h5-12,18H,1-4H3;1H

InChI key

KSCQDDRPFHTIRL-UHFFFAOYSA-N

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General description

Certified for use by fluorescence microscopy in Churukian′s modification of Truant′s fluorescent method for acid fast bacilli on paraffin sections.

Auramine O is used for the staining of acid-fast organisms like coccidia. Along with carbol, it generates brilliant yellow fluorochrome of tubercle bacilli. It is effective in detecting positive cases of tuberculosis. Auramine O binds to the mycolic acid in the bacterial cell wall.

Application

Auramine O has been used to study its absorption by a natural biopolymer poly (γ-glutamic acid).

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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E W Koneman.
Koneman's Color Atlas and Textbook of Diagnostic Microbiology, 1565-1565 (2006)
Equilibrium and kinetic studies on sorption of basic dyes by a natural biopolymer poly (γ-glutamic acid).
Inbaraj B S, et al.
Biochemical Engineering Journal, 31(3), 204-215 (2006)
E Kohen.
Cell Structure and Function by Microspectrofluorometry, 490-490 (2014)
M Habtamu et al.
The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease, 16(3), 402-407 (2012-05-30)
Mawenzi Regional Hospital, northern Tanzania. To determine the value of light-emitting diode (LED) microscopy in diagnosing tuberculosis (TB) on bleach-treated and direct sputum smears. Sputum samples were collected from patients suspected of pulmonary TB who presented consecutively at the laboratory
Ismael A Heisler et al.
The journal of physical chemistry. B, 113(6), 1623-1631 (2009-01-17)
The effects of confinement on the ultrafast torsional reaction of auramine O in aqueous solution are investigated through ultrafast fluorescence up-conversion with 50 fs time resolution. The aqueous solution is confined in nanoscale water droplets by an ionic surfactant. The
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