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A9207

Sigma-Aldrich

Avidin−Agarose from egg white

aqueous glycerol suspension

Sinónimos:

Avidin agarose

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1 ML
MXP 3,465.00
5 ML
MXP 11,896.00

MXP 3,465.00

Fecha estimada de envío31 de marzo de 2025

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1 ML
MXP 3,465.00
5 ML
MXP 11,896.00

About This Item

MDL number:
MFCD00130572
UNSPSC Code:
41106514
NACRES:
NA.56

MXP 3,465.00

Fecha estimada de envío31 de marzo de 2025

biological source

egg white

Quality Level

200

conjugate

agarose conjugate

form

aqueous glycerol suspension

matrix

cross-linked 6% beaded agarose

matrix activation

epichlorohydrin

matrix attachment

amino

matrix spacer

15 atoms

storage temp.

−20°C

Categorías relacionadas

General description

Avidin comprises of 0.05% of total egg white protein. It is a glycosylated protein. It corresponds to a molecular weight of 16.5 kDa and exists as tetramer, which can bind a minimum of three biotin molecules.[1]

Application

Avidin is used for the purification of biotin containing proteins, which can enhance cellular binding and internalization of the antibody (Ab)-functionalized capsules compared with control human immunoglobulin (IgG)-functionalized capsules, suggesting these capsules can specifically interact with cancer cells through antibody/antigen recognition.
Avidin-Agarose from egg white has been used:
  • in the precipitation of biotin tagged protein by pull down assay in neuroblastoma cell line (SH-SY5Y )[2]
  • in the purification of Lewis(x) sugar binding proteins from Schistosoma mansoni[3]
  • in avidin bead assay for purification of labelled biotin[4]
  • for capture of glycoproteins from murine cornea samples[5]

Biochem/physiol Actions

Avidin finds biotechnological applications due to its high-affinity ligand binding property. Avidin interaction with biotin through its carbohydrate chains is widely exploited in biotechnological applications.[1] In cancer cells, avidin-biotin systems helps in the in vivo localization and drug targeting in tumor cells.[6]

Unit Definition

One unit will bind 1.0 μg of d-biotin.

Physical form

Suspension in 50% glycerol, 0.01 M sodium phosphate, pH 7.0, containing 0.02% sodium azide.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000322714
0000255119
0000292850
0000247147
SLCH2002

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Covalent modification of cytoskeletal proteins in neuronal cells by tryptamine-4, 5-dione
Kato Y, et al.
Redox Biology, 2, 983-990 (2014)
Amber L Jolly et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 31(6), 2393-2404 (2017-02-23)
Cell surface glycosylation is thought to be involved in barrier function against microbes at mucosal surfaces. Previously we showed that the epithelium of healthy mouse corneas becomes vulnerable to
Dielectric spectroscopy of molecular interactions based on the avidin-biotin complex
Mellor, B.L., et al.
Conference proceedings : Annual International Conference of the IEEE Engineering in Medicine and Biology Society, 3660-3663 (2011)
Characterization of poultry egg-white avidins and their potential as a tool in pretargeting cancer treatment
Hytonen VP, et al.
The Biochemical Journal, 372(1), 219-225 (2003)
Jens Spross et al.
Analytical and bioanalytical chemistry, 402(7), 2395-2405 (2012-01-21)
A poly(glycidyl methacrylate-co-acrylamide-co-ethylene dimethacrylate) monolith and a poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolith were prepared in fused silica capillaries (100 μm ID) and modified with monomeric avidin using the glutaraldehyde technique. The biotin binding capacity of monolithic affinity columns with immobilized monomeric
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Contenido relacionado

Técnicas de análisis pull-down de proteínas

Investigue las interacciones proteína-proteína in vitro con análisis pull-down, utilizando métodos de afinidad, pull-down con GST, TAP y co-inmunoprecipitación.

Protein Pull-Down Techniques

Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

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