A8353

Anti-hABH3 antibody, Mouse monoclonal

clone hABH3-99, purified from hybridoma cell culture

• Sinónimos: Anti-Human AlkB Homologue 3, Monoclonal Anti-hABH3 antibody produced in mouse
• Número MDL: MFCD06412252
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• conjugado: unconjugated
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: hABH3-99, monoclonal
• Formulario: buffered aqueous solution
• mol peso: antigen ~33 kDa
• reactividad de especies: rat, hamster, monkey, bovine, canine, human, mouse
• técnicas: immunohistochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; microarray: suitable; western blot: 1-2 μg/mL using total cell extract of 293T cells
• isotipo: IgG1
• Nº de acceso UniProt: Q96Q83
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: Monkey ... ALKBH3(715921) ; human ... ALKBH3(221120) ; mouse ... Alkbh3(69113) ; rat ... Alkbh3(362169)

Descripción

Descripción general

Human alkB homolog 3, alpha-ketoglutarate dependent dioxygenase (hABH3) is mainly found in the nucleoplasm and cytoplasm.[1] Anti-hABH3 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hABH3-99 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with hABH3, conjugated to keyhole limpet hemocyanin (KLH).

Especificidad

Mouse Monoclonal Anti-hABH3 binds to human, monkey, bovine, dog, rat, hamster, and mouse hABH3. It does not recognize hABH1 or hABH2. The antibody epitope resides within amino acids 2-16 of human hABH3.

Aplicación

• Mouse Monoclonal Anti-hABH3 has been used in:
• western blot
• immunohistochemistry[1]
• immunoprecipitation
• indirect enzyme linked immunosorbent assay (ELISA)

Mouse Monoclonal Anti-hABH3 can be used for western blot (1-2μg/mL), immunohistochemistry, immunoprecipitation, indirect ELISA and microarray studies.

Acciones bioquímicas o fisiológicas

Human alkB homolog 3, α-ketoglutarate dependent dioxygenase (hABH3) is involved in the repair of alkylated RNA. This antibody serves as an important tool for studying the regulation of DNA/RNA repair enzymes.

hABH3 is a Fe(II)-dependent dioxygenase involved in the repair of alkylation-induced damage in DNA. hABH3 mutations have been analysed in pediatric brain tumors.
Mouse Monoclonal Anti-hABH3 binds to human, monkey, bovine, dog, rat, hamster, and mouse hABH3. It does not recognize hABH1 or hABH2. The antibody epitope resides within amino acids 2-16 of human hABH3.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): nwg
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable