94310

Uricase from Bacillus fastidiosus

lyophilized, ~9 U/mg

• Sinónimos: Urate oxidase
• Número de CAS: 9002-12-4
• Comisión internacional de enzimas: 1.7.3.3 (BRENDA, IUBMB)
• Número MDL: MFCD00082126
• Código UNSPSC: 12352204
• ID de la sustancia en PubChem: 57653596
• NACRES: NA.54

Propiedades

• origen biológico: Bacillus sp. (B. fastidiosus)
• Formulario: powder
• calidad: lyophilized
• actividad específica: ~9 U/mg
• condiciones de almacenamiento: dry at room temperature
• concentración: 90—100%
• color: white to faint beige
• solubilidad: 5 mg/mL, clear, colorless to very faintly yellow (in enzyme dilution buffer)
• aplicaciones: detection
• temp. de almacenamiento: −20°C
• cadena SMILES: N.OCC1OC(C(O)C1OP(O)(=O)OCC2OC(C(O)C2O)N3C=CC(=O)NC3=O)N4C=CC(=O)NC4=O
• InChI: 1S/C18H23N4O14P.H3N/c23-5-7-14(13(28)16(34-7)22-4-2-10(25)20-18(22)30)36-37(31,32)33-6-8-11(26)12(27)15(35-8)21-3-1-9(24)19-17(21)29;/h1-4,7-8,11-16,23,26-28H,5-6H2,(H,31,32)(H,19,24,29)(H,20,25,30);1H3
• Clave InChI: VPXDHZMLJOJKOX-UHFFFAOYSA-N

Descripción

Descripción general

Research area: Cell signaling

Uricase is present in many organisms, such as, archaea, bacteria, and eukaryotes. The Gut microbiome is the major source of uricase, a homotetrameric enzyme containing four identical 34 kDa subunits.

Aplicación

Uricase may be used for the determination of uric acid in biological fluids. It may be helpful in the spectrophotometric determination of purines and purine nucleosides.

Acciones bioquímicas o fisiológicas

Uricase catalyzes the oxidation of uric acid to 5-hydroxyisourate, which subsequently forms (S)-allantoin. It is involved in purine metabolism, urate degradation and (S)-allantoin formation [1]The absence of functional uricase in humans has significant physiological and pathophysiological implications. It has been associated with higher serum uric acid levels, leading to an increased risk of conditions related to uric acid crystallization, such as gout. Uricase has emerged as an important therapeutic target for the treatment of conditions related to hyperuricemia, such as gout.

Definición de unidad

1 U corresponds to the amount of enzyme which oxidizes 1 μmole uric acid at pH 9.1 and 37 °C.

Otras notas

For the determination of uric acid in biological fluidscitation; In the spectrophotometric determination of purines and purine nucleosidescitation.

For the determination of uric acid in biological fluids[2]; In the spectrophotometric determination of purines and purine nucleosides[3].

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H319,H334,H360FD
• Consejos de prudencia: P202 - P261 - P264 - P280 - P305 + P351 + P338 - P308 + P313
• Clasificaciones de peligro: Eye Irrit. 2 - Repr. 1B - Resp. Sens. 1
• Código de clase de almacenamiento: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)