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76220

Sigma-Aldrich

Papain from Carica papaya

powder, ≥3 U/mg

Sinónimos:

Papain powder

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About This Item

Número de CAS:
9001-73-4
Comisión internacional de enzimas:
3.4.22.2 (BRENDA, IUBMB)
EC Number:
232-627-2
MDL number:
MFCD00131791
UNSPSC Code:
12352204
eCl@ss:
42030311
NACRES:
NA.54

biological source

papaya

form

powder

specific activity

≥3 U/mg

mol wt

Mr ~23000

storage temp.

2-8°C

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Application

Papain is used as a meat tenderizer. It′s also used to break up tissue in the preparation of primary cell cultures.

Biochem/physiol Actions

Papain is a cysteine protease that cleaves peptide bonds and is known to cleave the Fc portion of antibodies.

Unit Definition

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol N-benzoyl-L-arginine ethyl ester (BAEE, Cat. No. 12880) per minute at pH 6.2 and 25°C

Other Notes

An important cysteine protease ; Peptide synthesis

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Review: novel cysteine proteases of the papain family.
F Bühling et al.
Advances in experimental medicine and biology, 477, 241-254 (2000-06-13)
M Y Gololobov et al.
Biotechnology and bioengineering, 40(3), 432-436 (1992-07-01)
The published theory of kinetically controlled enzymatic peptide synthesis needed experimental verification. We carried out the measurement of the peptide yield and estimation of the key parameters alpha and beta for papain-catalyzed synthesis of Mal-L-Phe-L-Ala-LLeuNH(2) from Mal-L-Phe-L-AlaOMe and L-LeuNH(2). The
Catalytic mechanism in papain family of cysteine peptidases.
A C Storer et al.
Methods in enzymology, 244, 486-500 (1994-01-01)
Adrienne McMahon
Journal of AOAC International, 99(1), 223-241 (2016-02-10)
The main objective of the AOAC Stakeholder Panel on Infant Formula and Adult Nutritionals (SPIFAN) project is to establish international consensus methods for infant formula and adult nutritionals, which will benefit intermarket supply and dispute resolution. A collaborative study was
Chih-Lun Su et al.
Communications biology, 5(1), 1166-1166 (2022-11-04)
Various magnetic deep brain stimulation (DBS) methods have been developing rapidly in the last decade for minimizing the invasiveness of DBS. However, current magnetic DBS methods, such as magnetothermal and magnetomechanical stimulation, require overexpressing exogeneous ion channels in the central
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