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51290

Sigma-Aldrich

Hemoglobin from bovine blood

lyophilized, 2×cryst., ≥90% (SDS-PAGE), powder

Sinónimos:

Bovine hemoglobin, Hb, Methemoglobin

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About This Item

Número de CAS:
9008-02-0
MDL number:
MFCD00131282
UNSPSC Code:
12352202

biological source

bovine blood

assay

≥90% (SDS-PAGE)

form

powder

quality

2×cryst.
lyophilized

mol wt

Mr ~64500

technique(s)

UV/Vis spectroscopy: suitable

impurities

salt, none detected
~0.3% Fe

loss

≤8% loss on drying

UniProt accession no.

P01966
P02070
P02081
P06642
P06643

storage temp.

2-8°C

Gene Information

cow ... HBA1(281221) , HBB(280813) , HBE2(513240) , HBE4(513108) , HBG(511735) , alpha globin(512439)

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General description

Hemoglobin (Hb) is the protein responsible for storage and transport of oxygen and other gaseous ligands in red blood cells (RBC). Hb is also the pioneer material for synthesis of Hb-based oxygen carriers (HBOCs) that can be used as substitutes for RBC.

Application

The solubility of α-elastin has been applied to construction of elastin-mimetic biomaterials.

Biochem/physiol Actions

Oxygen transporter. The Fe2+/Fe3+ balance is a physiological indicator of blood oxygenation; deoxygenated hemoglobin accessorizes a feedback loop by reducing nitrite to NO, a vasodilator which enhances blood flow to oxygen-deprived tissues.

Caution

Since native hemoglobin is readily oxidized in air, these preparations may be predominantly methemoglobin.

Other Notes

Review: Oxygen carrier proteins
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Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Paul W Buehler et al.
Biomaterials, 31(13), 3723-3735 (2010-02-13)
Hemoglobin-based oxygen carriers (HBOC) are currently being developed as red blood cell (RBC) substitutes for use in transfusion medicine. Despite significant commercial development, late stage clinical results of polymerized hemoglobin (PolyHb) solutions hamper development. We synthesized two types of PolyHbs
M. Brunori et al.
Topics in Molecular and Structural Biology, 7, 263-263 (1985)
Xiao-Su Hu et al.
Journal of biomedical optics, 18(1), 17003-17003 (2013-01-08)
The reduction of trial-to-trial variability (TTV) in task-evoked functional near-infrared spectroscopy signals by considering the correlated low-frequency spontaneous fluctuations that account for the resting-state functional connectivity in the brain is investigated. A resting-state session followed by a task-state session of
Toru Yamada et al.
PloS one, 7(11), e50271-e50271 (2012-11-28)
In conventional functional near-infrared spectroscopy (fNIRS), systemic physiological fluctuations evoked by a body's motion and psychophysiological changes often contaminate fNIRS signals. We propose a novel method for separating functional and systemic signals based on their hemodynamic differences. Considering their physiological
Omar Falou et al.
Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference, 2012, 3155-3158 (2013-02-01)
The necessity for a non-invasive and inexpensive imaging modality to both diagnose and monitor treatment response has lead to renewed interest in the potential of optical imaging. The aim of this study was to investigate the potential of diffuse optical
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Chromatograms

application for HPLC

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