41155

Anti-Rabbit IgG−Abberior^® CAGE 500 antibody produced in goat

for single-molecule switching microscopy (e.g. PALM, STORM, GSDIM)

• Sinónimos: Abberior^® CAGE 500P-Anti-Rabbit IgG antibody produced in goat
• UNSPSC Code: 12352200
• NACRES: NA.45

Propiedades

• biological source: goat
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: rabbit
• availability: available only in USA and Canada
• concentration: ~1 mg/mL
• fluorescence: λ_ex 511 nm; λ_em 525 nm in PBS, pH 7.4 (photoactivated)
• suitability: corresponds for SDS-PAGE
• storage temp.: −20°C

Descripción

General description

Abberior CAGE 500 is a caged dye which is initially colorless and nonfluorescent. When illuminated with UV light it undergoes a rapid uncaging reaction and releases a highly fluorescent dye which fluoresces at 511 nm.
Abberior CAGE 500 is designed for single-molecule photoswitching microscopy modes such as PALM, STORM and GSDIM and, in its uncaged form, also performs well in STED microscopy. It performs very well with Leica GSD microscope and other superresolution microscopes such as Nikon N-Storm, particularly as a 2 Color partner with CAGE 552 or FLIP 565.
Further, after photoactivation, Abberior CAGE 500 can be tracked to analyze molecular dynamics such as diffusion, flow directions and velocities (e.g. are tracking experiments in which the fluorescence signal is observed over time after the initial release of the fluorophore).


Photophysical properties (carboxylic acid):
Absorption Maximum, λ_ex [nm]: 511 (photoactivated, PBS pH 7.4), 299 (non-photoactivated, PBS pH 7.4)
Extinction Coefficient, ε_max [M^-1cm^-1]: 50 000 (photoactivated, PBS pH 7.4)
Photoactivation Wavelength, λ [nm]: 360-440
Fluorescence Maximum, λ_em [nm]: 525 (photoactivated, PBS pH 7.4)
Recommended STED Wavelength, λ [nm]: 590-610
Fluorescence Quantum Yield, λ: 0,85 (photoactivated, PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 4,1 (photoactivated, PBS pH 7.4)

Suitability

Designed and tested for fluorescent super-resolution microscopy

Analysis Note

May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence

Other Notes

Find more infomation here Abberior^®

Legal Information

abberior is a registered trademark of Abberior GmbH

Información de seguridad

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable