The recommended procedure for a 5-minute incubation in 1X Citrate Phosphate Buffer solution should be followed, with adjustments made based on the degree of staining observed with the slides. The slides should be agitated at 1 and 3 minutes, and the user should determine the amount of agitation required based on their observations. The degree of agitation may need to be adjusted to address issues such as hard-to-observe ghost cells or an excess of adult hemoglobin.
2851
Citrate Phosphate Buffer Concentrate
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NACRES:
NA.47
UNSPSC Code:
41116124
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solution
Quality Segment
IVD
for in vitro diagnostic use
application(s)
hematology
histology
shipped in
wet ice
storage temp.
2-8°C
Application
- Poly(sulfobetaine) versus poly(ethylene glycol) based copolymer modified polyurethane catheters for antifouling: This study examines the use of ethanol in the synthesis of polymers designed to reduce biological fouling on medical devices, highlighting its role in advanced healthcare materials research (Cao et al., 2024).
- Green synthesis of polyimide by using an ethanol solvothermal method for aqueous zinc batteries: Ethanol is used as a green solvent in the innovative synthesis of polyimide structures, emphasizing its importance in sustainable material production and energy storage solutions (Zhao et al., 2024).
- Modulation of gut microbiota with probiotics as a strategy to counteract endogenous and exogenous neurotoxicity: Research explores the indirect applications of ethanol in the solvent extraction processes used for studying neuroprotective strategies in medical research (Skalny et al., 2024).
- Superselective Ablative Chemo-ethanol Embolization for Recurrent Single Hepatocellular Carcinoma: A Six-Month Outcome Analysis: This clinical study utilizes ethanol directly in therapeutic interventions, demonstrating its critical use in oncological treatments to improve patient outcomes (Lee et al., 2024).
- Self-Assembly Reinforced Alginate Fibers for Enhanced Strength, Toughness, and Bone Regeneration: Ethanol is employed in the processing of novel biomaterials designed for medical applications, showcasing its utility in the development of regenerative medicine technologies (Cui et al., 2024).
Preparation Note
Sodium citrate, 0.7 mol/L, and sodium phosphate, 0.6 mol/L
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Warning
hcodes
Hazard Classifications
Eye Irrit. 2
Clase de almacenamiento
10 - Combustible liquids
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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Is it advisable to switch to the published procedure, which requires a 5-minute incubation, considering the change in cell appearance after immersing the slides for 2 minutes in the buffer?
1 answer-
Helpful?
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Are adult red blood cells failing to properly elute hemoglobin after incubation in the Citrate Phosphate Buffer working solution? Could the pH of the Citrate Phosphate Buffer be too high? Additionally, is it feasible to increase the amount of agitation to aid in eluting the adult hemoglobin?
1 answer-
To elute adult hemoglobin, pure/undenatured 80% alcohol is necessary. The use of histological or reagent grade alcohol may result in inadequate elution.
Helpful?
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