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14420C

SAFC

EX-CELL® 420 Serum-Free

with L-glutamine, sterile-filtered, suitable for insect cell culture

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About This Item

MDL number:
MFCD01634638
UNSPSC Code:
12352207
NACRES:
NA.75

description

for research or for further manufacturing use

sterility

sterile-filtered

product line

EX-CELL®

form

liquid

technique(s)

cell culture | insect: suitable

components

HEPES: no
L-glutamine: yes
glucose: yes
NaHCO3: yes

shipped in

ambient

storage temp.

2-8°C

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Application

EX-CELL 420 is a complete medium developed and optimized for the serum-free growth of Sf9 and Sf21 insect cell lines. Cells can be subcultured directly into EX-CELL 420 from serum-free or serum-supplemented media without adaptation. Cultures in EX-CELL 420 routinely reach cell densities greater than 1 × 107 cells/mL with greater than 95% viability. Suspension cultures can be maintained, without refeeding, for more than 10 days. Sf9 and Sf21 cells have been carried for more than 20 passages in EX-CELL 420 with no loss of viability. Protein expression and virus production are improved over serum-containing media.
EX-CELL 420 is a complete medium developed and optimized for the serum-free growth of Sf9 and Sf21 insect cell lines. Cells can be subcultured directly into EX-CELL 420 from serum-free or seum-supplemented media without adaptation. Cultures in EX-CELL 420 routinely reach cell densities greater than 1 × 107 cells/mL with greater than 95% viability. Suspension cultures can be maintained, without refeeding, for more than 10 days. Sf9 and Sf21 cells have been carried for more than 20 passages in EX-CELL 420 with no loss of viability. Protein expression and virus production are improved over serum-containing media.

Legal Information

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

Comparable product

Referencia del producto
Descripción
Precios

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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T3160

TC-100 Insect Medium

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I7267

Insect Medium Supplement (10×)

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T3285

TNM-FH insect medium

Norikatsu Nishikawa et al.
Cytotechnology, 43(1-3), 3-10 (2008-11-13)
The production of beta-galactosidase by Sf9 cells infected with recombinant Autographa californica nucleopolyhedrovirus (AcNPV) was investigated in shake-flask culture using two serum-free basal media: Grace's medium and TNM-FH (Grace's medium supplemented with lactalbumin hydrolysate and yeast extract). At the time
Fabiana R X Batista et al.
Cytotechnology, 49(1), 1-9 (2008-11-13)
Animal cells can be cultured both in basal media supplemented with fetal bovine serum (FBS) and in serum-free media. In this work, the supplementation of Grace's medium with a set of nutrients to reduce FBS requirements in Spodoptera frugiperda (Sf9)
Hideki Yamaji et al.
Journal of bioscience and bioengineering, 95(2), 185-187 (2005-10-20)
When rat protein kinase C-delta (PKC-delta) was produced by Sf9 cells infected with a recombinant baculovirus in shake-flask culture using a serum-containing medium, the intracellular PKC-delta content decreased in the late period while the extracellular PKC-6 markedly increased. During the

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