01494

Nancy-520

suitable for RT-PCR, ≥90.0% (HPCE)

• UNSPSC Code: 12171500
• EC Number: 200-664-3
• NACRES: NA.32

Propiedades

• Quality Segment: 100
• assay: ≥90.0% (HPCE)
• form: liquid
• mol wt: 78.13 g/mol
• sustainability: Sustainability features
• color: red to very dark red
• bp: 189 °C/1,013 hPa
• mp: 18.4 °C
• solubility: DMSO: soluble
• density: 1.104 g/cm³ at 20 °C
• fluorescence: λ_ex 520 nm; λ_em 554 nm in TE buffer; DNA
• storage temp.: 2-8°C

Descripción

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product was Designed for Safer Chemistry

Application

Nancy-520 is a fluorescent stain for dsDNA on agarose electrophoresis gels, with higher sensitivity than ethidium bromide and an easy, fast, and robust staining procedure. It is provided as a 5000x stock solution (500 μl), which is sufficient for 50 agarose gels. The limit of detection is 0.5 ng/band of dsDNA.
In addition, Nancy-520 can be used to determine dsDNA concentrations in solution, with a linear range between 0 - 2 μg/ml of DNA. Nancy 520

Nancy-520 has been used successfully in PCR (Real time-PCR) applications.
Nancy 520

Greener alternative to Ethidium bromide.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Información de seguridad

• Clase de almacenamiento: 10 - Combustible liquids
• ¿Qué está pasando?: WGK 2
• Punto de inflamación (°F): 188.6 °F - closed cup - Solvent
• Punto de inflamación (°C): 87 °C - closed cup - Solvent
• EPI (equipos de protección individual): Eyeshields, Gloves, type ABEK (EN14387) respirator filter