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Merck

270733

Sigma-Aldrich

Agua

Water
1 of 1 reviewers received a sample product or took part in a promotion

suitable for HPLC

Sinónimos:

Aqua, Óxido de dihidrógeno

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1 L
MXP 1,571.00
4 L
MXP 3,500.00
4 X 4 L
MXP 11,628.00
20 L
MXP 13,784.00

MXP 1,571.00


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Seleccione un Tamaño

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1 L
MXP 1,571.00
4 L
MXP 3,500.00
4 X 4 L
MXP 11,628.00
20 L
MXP 13,784.00

About This Item

Fórmula lineal:
H2O
Número de CAS:
Peso molecular:
18.02
Beilstein/REAXYS Number:
2050024
EC Number:
MDL number:
UNSPSC Code:
12190000
PubChem Substance ID:
NACRES:
NA.21
technique(s):
HPLC: suitable
bp:
100 °C (lit.)
vapor pressure:
3 mmHg

MXP 1,571.00


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vapor density

<1 (vs air)

Quality Level

vapor pressure

3 mmHg

form

liquid

technique(s)

HPLC: suitable

evapn. residue

<0.0003%

refractive index

n20/D 1.34 (lit.)

pH

6.0-8.0 (25 °C)

bp

100 °C (lit.)

mp

0 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

λ

H2O reference

UV absorption

λ: 205 nm Amax: 0.01
λ: 210 nm Amax: 0.01
λ: 250-400 nm Amax: 0.005

SMILES string

O

InChI

1S/H2O/h1H2

InChI key

XLYOFNOQVPJJNP-UHFFFAOYSA-N

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General description

El agua adecuada para HPLC suele utilizarse como disolvente para la elución isocrática en la cromatografía de cationes y de aniones. Además, se utiliza como un componente para preparar disoluciones patrón de reserva, y para la evaporación bajo una corriente de nitrógeno en un baño de agua para concentrar la muestra antes del análisis mediante LC-MS-MS.[1] [2]

Application

El agua se utiliza como:
  • Disolvente para preparar disoluciones tampón durante el proceso de lisis celular.[3]
  • Componente de la fase móvil durante la detección mediante HPLC–UV–matriz de diodos para la separación y la detección de los analitos.[4]

Preparation Note

Producto filtrado a través de un filtro de 0,2 μm

Other Notes

Aviso importante
El artículo de referencia 270733-6X1L será descatalogado. Pida la botella 270733-1L que es físicamente idéntica con las mismas especificaciones exactas.

Recommended products

Descubra los reactivos LiChropur ideales para análisis mediante HPLC o LC-MS

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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Certificados de análisis (COA)

Lot/Batch Number

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Protocolos

A guide to create solvent systems used for the thin-layer chromatography assay of Novabiochem products.

Questions

1–6 of 6 Questions  
  1. Do you know the resistivity value @ 25°C?

    1 answer
    1. The resistivity of this product has not been determined.

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  4. How is this water purified? is it distilled?

    1 answer
    1. The water purification process involves several steps:
      • The water initially passes through a carbon exchange tank to remove organic compounds.
      • It is then filtered using a 1μm PE cartridge filter to remove any remaining impurities.
      • Inorganic compounds are eliminated through deionization tanks, ensuring high purity. The resistivity of the water is checked during this stage.
      •The water undergoes sterilization using UV light to eliminate any microorganisms.
      • Finally, the water is filtered through a 0.2μm cartridge filter, and the resulting HPLC grade water is packaged in glass bottles.
      Note: This information provides an overview of the typical water purification process and may vary depending on specific applications or systems.

      Helpful?

  5. Do you know the concentration of TOC in this water?

    1 answer
    1. The concentration of TOC in this product is not determined. Product 78533, Water for TOC Analysis, may be of interest.
      Please see the link below to review this product option:
      https://www.sigmaaldrich.com/product/sial/78533

      Helpful?

  6. Is this product (HPLC grade water #270733) DNase-, RNase- and protease-free?

    1 answer
    1. The item is not tested for the presence of DNA, RNA, and protease.

      Please see the links below to review product options that include this testing:
      W4502
      https://www.sigmaaldrich.com/US/en/product/sigma/w4502
      95284
      https://www.sigmaaldrich.com/US/en/product/sigma/95284

      Note that the above options are NOT tested for suitability in HPLC.

      Helpful?

Reviews

1 of 1 reviewers received a sample product or took part in a promotion

Active Filters

    • Reviews 3
    • Votes 9
    5 out of 5 stars.

    Good, pure water

    Water was high purity, HPLC grade. We didn't actually use it for HPLC but it seemed very clean and pure for our purposes.

    Helpful?

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