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Merck

I3021

Sigma-Aldrich

IGEPAL® CA-630

viscous liquid

Sinónimos:

Octylphenoxy poly(ethyleneoxy)ethanol, branched

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About This Item

Fórmula lineal:
(C2H4O)nC14H22O
Número de CAS:
UNSPSC Code:
12161900
NACRES:
NA.21

description

non-ionic

Quality Level

form

viscous liquid

mol wt

~603

CMC

0.08 mM (20-25°C)

viscosity

250-350 cP, neat(25 °C)(lit.)

density

1.06 g/cm3 at 25 °C (lit.)

HLB

13

InChI

1S/C16H26O2/c1-15(2,3)12-16(4,5)13-6-8-14(9-7-13)18-11-10-17/h6-9,17H,10-12H2,1-5H3

InChI key

JYCQQPHGFMYQCF-UHFFFAOYSA-N

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General description

IGEPAL® CA-630 is a nonionic, non-denaturing detergent suitable for solubilization, isolation and purification of membrane protein complexes. It is chemically indistinguishable from Nonidet P-40.

Application

IGEPAL® CA-630 has been used:
  • as a component of grinding buffer along with lithium dodecyl sulphate, tris-HCl, EDTA, NaCl, sodium deoxycholate, dithiothreitol and thiourea in RNA extraction process
  • along with Tris- HCl in incubation of Tachyzoites cultured in vero cells
  • as a component of nuclear extract buffer D+ containing HEPES, glycerol, KCl and EDTA for gel shift analysis
  • as a component of PI buffer along with NaH2PO4 to wash slides in FISH (fluorescence in situ hybridization) procedure
  • for solubilizing and isolation of membrane proteins

Other Notes

Nonionic, non-denaturing detergent

Quality

Chemically indistinguishable from Nonidet P-40, which is no longer commercially available.

Caution

May develop turbidity or sediment during storage. A clear liquid can be obtained on heating to 40°C.

Legal Information

IGEPAL is a registered trademark of Solvay

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

483.8 °F - closed cup

flash_point_c

251 °C - closed cup

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


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Protocolos

This page shows how to solubilize membrane proteins with products from Cytiva.

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