Toluidine Blue is used in various staining procedures, and preparation may vary depending on the specific procedure. For staining frozen sections, aqueous solutions with concentrations ranging from 0.3% to 1% can be used. Additionally, a 0.5% dye in 20% alcohol is suitable for frozen sections. Other solutions may require a specific pH or special preparation.
89640
Toluidine Blue
8.74% (ZN (THEORY)), for microscopy (Hist., Vit.)
Sinónimos:
Tolonium chloride; Basic Blue 17
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About This Item
Fórmula empírica (notación de Hill):
C15H16ClN3S · 0.5ZnCl2
Número de CAS:
Peso molecular:
373.97
Número de índice de color:
52040
Beilstein:
4068052
Número MDL:
Código UNSPSC:
12171500
ID de la sustancia en PubChem:
NACRES:
MA.02
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Productos recomendados
grado
for microscopy (Hist., Vit.)
Nivel de calidad
Ensayo
8.74% (ZN (THEORY))
Formulario
powder or crystals
técnicas
microbe id | staining: suitable
color
green to very dark green
solubilidad
methanol: 0.01 g/10 mL, blue to very deep blue
εmax
≥800 at 621-636 nm in water
idoneidad
suitable for microscopy
aplicaciones
diagnostic assay manufacturing
hematology
histology
temp. de almacenamiento
room temp
cadena SMILES
[Cl-].[Cl-].Cl[Zn]Cl.Cc1cc2N=C3C=CC(\C=C3Sc2cc1N)=[N+](\C)C.Cc4cc5N=C6C=CC(\C=C6Sc5cc4N)=[N+](\C)C
InChI
1S/2C15H15N3S.4ClH.Zn/c2*1-9-6-13-15(8-11(9)16)19-14-7-10(18(2)3)4-5-12(14)17-13;;;;;/h2*4-8,16H,1-3H3;4*1H;/q;;;;;;+2/p-2
Clave InChI
DAQXTSXITPUGRG-UHFFFAOYSA-L
Categorías relacionadas
Descripción general
Toluidine blue (C.l. 52040) is a diaminothiazine dye.It is also known as TBO, tolonium chloride, and toluidine blue O.
Aplicación
Toluidine Blue is suitable for use in the following applications:
- to demonstrate amyloid; DNA in plant material, mast cells, Nissl substance, nuclei, and sulfatides
- in metachromatic staining of sulfated mucins and related materials
- as staining agent to identify the development and accumulation of the cartilage matrix, proteoglycans for isolation and culture of mesenchymal stem cells from mouse compact bone.
- staining of mucins in water-miscible resins and of nucleic acids in epoxy resins(1)
Acciones bioquímicas o fisiológicas
Toluidine Blue is a basic thiazine metachromatic dye, which is used for selectively staining acidic tissue components such as carboxylates, sulfates, and phosphate radicals.It is found to have an affinity for nucleic acids and therefore binds to the nuclear material of tissues with a high DNA and RNA content.
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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Are there any instructions on how to mix Toluidine Blue powder (89640) into a staining solution?
1 answer-
Helpful?
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What does C.l. 52040 mean?
1 answer-
The C.I. number is the Colour Index number. This number is globally recognized and is assigned to Dyes and Pigments by the Americal Association of Textile Chemists and Colourists (AATCC) established in 1924. This compound is assigned a C.I. number of 52040. Please see the link below to learn more about the Colour Index program:
https://colour-index.com/Helpful?
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