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3HBDB-RO

Roche

3-Hydroxybutyrate Dehydrogenase (3-HBDH)

Name: 3-Hydroxybutyrate Dehydrogenase (3-HBDH)
Brand: ROCHE

suspension, ~3 units/mg protein (At 25 °C with 3-hydroxybutyrate as the substrate; standardized with BSA.), grade II

Sinónimos:

3-Hydroxybutyrate Dehydrogenase (3-HBDH), 3-HBDH

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UNSPSC Code:

12352204

Número CE:

1.1.1.30 (BRENDA, IUBMB)

NACRES:

NA.54

Specific activity:

~3 units/mg protein (At 25 °C with 3-hydroxybutyrate as the substrate; standardized with BSA.)

Concentration:

5 mg/mL

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Propiedades

form

suspension

specific activity

~3 units/mg protein (At 25 °C with 3-hydroxybutyrate as the substrate; standardized with BSA.)

packaging

vial of 2 mL (10127833001), vial of 5 mL (10127841001)

manufacturer/tradename

Roche

concentration

5 mg/mL

impurities

<0.1% LDH, <5% MDH

optimum pH

6.2-6.9(for reduction), 7-9(for oxidation)

shipped in

wet ice

storage temp.

2-8°C

Descripción

General description

3-Hydroxybutyrate dehydrogenase (3-HBDH) enzyme, obtained from Rhodobacter sphaeroides, is generally used for the quantification of ketone bodies, such as D-3-hydroxybutyrate and acetoacetate.

Application

3-Hydroxybutyrate Dehydrogenase (3-HBDH) oxidizes selectively (R)-3-hydroxymonocarboxylic acids, or reverse reaction.

Biochem/physiol Actions

3-Hydroxybutyrate dehydrogenase (3-HBDH), a mitochondrial enzyme, catalyzes the reversible oxidation of 3-hydroxybutyrate (3HB) to acetoacetate, with NAD as coenzyme. In mammals, acetyl-coA is metabolized, in one of two pathways, to produce acetoacetate and D-3-hydroxybutyrate, which along with acetone are known as ketone bodies. 3-HBDH reversibly reduces this free acetoacetate to D-3-hydroxybutyrate. In patients with diabetic ketoacidosis (DKA), the ratio of 3HB and acetoacetate can be as high as 10:1, as compared to the normal ratio of 1:1. 3-HBDH enzyme can be used to detect the quantity of 3HB in urine, serum, and blood samples.

Physical form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Preparation Note

Stabilizers: NADH, Ca²⁺

Analysis Note

Contaminants: <0.1% LDH, <5% MDH

Other Notes

For life science research only. Not for use in diagnostic procedures.

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Este artículo	H9408	LLDHRO	H8509
Roche 3HBDBRO 3-Hydroxybutyrate Dehydrogenase (3-HBDH)	Sigma-Aldrich H9408 β-Hydroxybutyrate Dehydrogenase from Pseudomonas lemoignei	Roche LLDHRO L-Lactate Dehydrogenase (L-LDH)	Sigma-Aldrich H8509 β-Hydroxybutyrate Dehydrogenase from Rhodopseudomonas sphaeroides
description suspension, ~3 units/mg protein (At 25 °C with 3-hydroxybutyrate as the substrate; standardized with BSA.), grade II	description lyophilized powder, ≥200 units/mg protein	description -	description Type V, lyophilized powder, 250-750 units/mg protein
specific activity ~3 units/mg protein (At 25 °C with 3-hydroxybutyrate as the substrate; standardized with BSA.)	specific activity ≥200 units/mg protein	specific activity ~550 units/mg protein (at 25 °C (1,100 U/mg at 37 °C) with pyruvate as the substrate.)	specific activity 250-750 units/mg protein
form suspension	form lyophilized powder	form suspension	form lyophilized powder
concentration 5 mg/mL	concentration -	concentration -	concentration -
storage temp. 2-8°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C
shipped in wet ice	shipped in -	shipped in wet ice	shipped in -
optimum pH 7-9(for oxidation), 6.2-6.9(for reduction)	optimum pH -	optimum pH -	optimum pH -

Clase de almacenamiento

12 - Non Combustible Liquids

WGK

WGK 1

Punto de inflamación (°F)

No data available

Punto de inflamación (°C)