11388908910
Roche
Biotin-16-UTP
pkg of 25 μL (250 nmol; 10mM)
Sinónimos:
Biotin-16-UTP, biotin
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25 μL
MXP 5,761.00
MXP 5,761.00
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25 μL
MXP 5,761.00
About This Item
Código UNSPSC:
41116100
MXP 5,761.00
Check Cart for Availability
Productos recomendados
Ensayo
97.7% (HPLC)
Nivel de calidad
Formulario
solution
mol peso
Mr 987.5 (biotin-16-UTP-Li4)
envase
pkg of 25 μL (250 nmol; 10mM)
fabricante / nombre comercial
Roche
color
colorless
solubilidad
water: miscible
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
Descripción general
For biotin-16-UTP, biotin is bound to uridine triphosphate via an amide linkage. Biotin-16-UTP is a substrate for SP6, T3, and T7 RNA polymerase. It can replace UTP in the in vitro transcription reaction for RNA labeling.[1] Linearized template DNA with T7, SP6, or T3 promoter is in vitro-transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and Biotin-16-UTP, respectively.
Aplicación
Biotin-labeled RNA can be used as a hybridization probe for:
The labeled RNA can be subsequently detected with a fluorescent streptavidin conjugate or by enzyme linked immuno sorbent assay (ELISA) using a Streptavidin-AP conjugate.
The labeled RNA can be subsequently detected with a fluorescent streptavidin conjugate or by enzyme linked immuno sorbent assay (ELISA) using a Streptavidin-AP conjugate.
Calidad
Typical analysis: At least 85% Biotin-16-UTP (HPLC, area%).
Forma física
Biotin-16-UTP, tetralithium salt, 10mM solution
Otras notas
For life science research only. Not for use in diagnostic procedures.
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
does not flash
Punto de inflamabilidad (°C)
does not flash
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