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SCM307

Sigma-Aldrich

3dGRO® Lung Organoid Branching Medium

Serum-free ready-to-use media used to differentiate anterior foregut endoderm (AFE) cells into lung bud organoids in suspension cultures.

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.71
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Descripción general

The lung epithelium undergoes a complex series of endoderm–mesoderm-mediated signaling events to generate a complex arborized network of conducting airway (bronchi, bronchioles) and gas-exchanging units (alveoli). Lung epithelium differentiation stages include: anterior/posterior and dorsal/ventral patterning, lung specification, lung budding, branching morphogenesis, and maturation.
The 3dGRO® Lung Organoid Branching Medium is a chemically defined/serum-free ready-to-use media used to differentiate Sox2+/Pax6+ anterior foregut endoderm (AFE) cells into lung bud organoids in suspension cultures. Additionally, the 3dGRO® Lung Organoid Branching Medium can be used to culture AFE cells on fibronectin coated cultureware. The 3dGRO® Lung Organoid Branching Medium is a component of the 3dGRO® Human Lung Organoid Culture System which can be used to differentiate human iPS cells into mature lung organoids that structurally resemble the in vivo branching airway and early alveolar structures. The 3dGRO® Human Lung Organoid Culture System includes (all sold separately):

  • Definitive Endoderm Induction Medium (SCM302)
  • Anterior Foregut Endoderm Induction Medium I (SCM305)
  • Anterior Foregut Endoderm Induction Medium II (SCM306)
  • 3dGRO® Lung Organoid Branching Medium (SCM307)
  • 3dGRO® Lung Organoid Maturation Medium (SCM308)

Human iPSC Derived AFE Progenitors (SCC301)

Almacenamiento y estabilidad

Upon receipt, store at -20°C. When ready to use, thaw overnight at 2-8°C. Once thawed, mix thoroughly. Use immediately and store at 2-8°C for up to 1 week. Do not re-freeze. Unused aliquots may be stored at -20°C until the expiry date.

Información legal

3dGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 2

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

In Vitro Differentiation of Human iPS Cells into Airway Epithelial Lung Organoids for Respiratory Disease Research Applications

Lung organoids are valuable 3D models for human lung development and respiratory diseases. The 3dGRO™ differentiation protocol generates organoids from human iPSCs in 4 steps.

Organoid Culture Frequently Asked Questions

Organoid culture FAQs. Learn how to culture and analyze organoids following established 3D cell culture protocols. Tips and tricks.

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Questions

1–3 of 3 Questions  

  1. How are organoids cultured?

    1 answer

    1. Once isolated, organoids are cultured in specialized media embedded in a rich extracellular matrix (ECM) basement membrane extract hydrogel such as Growth Factor Reduced Matrigel. Media is replenished every other day and cells are passaged once per week (7-12 days) before cells become too large or necrotic. Cells can be passaged in either small clump fragments or as single cells using mechanical dissociation/enzyme-free passaging reagents. Depending on organoid type and confluency, split ratios of roughly 1:3-1:4 can be used when passaging organoids. For sensitive organoid types, ROCKi (Y27632) (SCM075) can be added during passaging to help in promoting cell viability.

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  2. How do you stain organoids with antibodies?

    1 answer

    1. Organoids can be stained with antibodies for immunocytochemical (ICC)/Immunohistochemical (IHC) analysis using either whole-mount or paraffin embedding/sectioning techniques. It is critical to use antibodies prequalified for organoid analysis.

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  3. How Do you Extract RNA/DNA from Organoids in Cultured in Matrigel?

    1 answer

    1. Traditional DNA/RNA isolation kits can be used to prepare genomic material for sequencing (RNA-seq, scRNA-seq, WGS, CHIP-seq etc). For some applications with large organoids, tissue specific DNA/RNA extraction kits are recommended. TRI Reagent (T9424) can be added to the dissociated organoids before RNA extraction and can be stored at -80 °C until future use.

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