This Normal Rabbit Serum is validated for use in ELISA, Flow Cytometry, Immunoblotting, Immunofluorescence, Immunohistochemistry, Immunoprecipitation for the detection of Rabbit Serum.
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Normal carrier sera from healthy, non-immunized rabbits.
Application
ELISA (see comments)
Flow Cytometry (see comments)
Immunoblotting (see comments)
Immunofluorescence (see comments)
Immunohistochemistry (see comments)
Immunoprecipitation (see comments)
Warning
Toxicity: Standard Handling (A)
Physical form
Serum diluted in 250 mM NaCl, 10 mM PBS, pH 7.6.
Reconstitution
Reconstitute the lypohilized diluted serum in 1 ml sterile distilled water with occasional gentle mixing for at least 2 h at 4°C. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) with 0.1% azide for short-term storage. Avoid freeze/thaw cycles of solutions.
Other Notes
Normal sera are provided to be used as blocking agents in immunohistochemical procedures or as negative controls for undiluted serum/rabbit polyclonal antibodies from the Calbiochem brand. For use as a negative control, perform experiments in parallel using primary antibody and normal serum at the same concentrations. A signal obtained with the normal serum indicates a nonspecific interaction; any additional signal with the primary antibody indicates a specific antibody-antigen interaction. This antibody has also been reported to work for ELISA, flow cytometry, immunoblotting, immunofluorescence, immunohistochemistry, and immunoprecipitation.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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