MBNMAG-41K

MILLIPLEX^® Mouse Bone Magnetic Bead Panel - Bone Metabolism Multiplex Assay

The analytes available for this multiplex kit are: ACTH, DKK-1, FGF-23, IL-6, Insulin, Leptin, Osteoprotegerin, SOST, TNF-α.

• UNSPSC Code: 12161503
• eCl@ss: 32161000
• NACRES: NA.84

Propiedades

• Quality Level: 200
• species reactivity: mouse
• manufacturer/tradename: Milliplex^®
• assay range: accuracy: 84-94%; standard curve range: 10-40,000 pg/mL; (Leptin); standard curve range: 15-60,000 pg/mL; (DKK-1); standard curve range: 16-65,000 pg/mL; (OPG); standard curve range: 3-12,000 pg/mL; (SOST); standard curve range: 3-12,000 pg/mL; (TNFα); standard curve range: 37-150,000 pg/mL; (Insulin); standard curve range: 4-15,000 pg/mL; (ACTH); standard curve range: 7-30,000 pg/mL; (FGF-23); standard curve range: 7-30,000 pg/mL; (IL-6)
• technique(s): multiplexing: suitable
• detection method: fluorometric (Luminex xMAP)
• shipped in: wet ice

Descripción

General description

Bone metabolism is the dynamic process of ongoing bone deposition and resorption, controlled by osteoblasts, osteocytes, and osteoclasts. While osteoblasts and osteocytes (osteoblasts surrounded by matrix) are responsible for bone deposition, osteoclasts are responsible for bone resorption. Both are required to maintain bone structure, as well as an adequate supply of calcium. To maintain this metabolic balance these cells rely on complex signaling pathways involving hormones and cytokines to achieve the appropriate rates of growth and differentiation. The disruption of bone metabolism results in such disease as osteoporosis, osteoarthritis, rheumatoid arthritis, chronic kidney disease and bone metastases.

MILLIPLEX^® Mouse Bone Panel is a 9-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma and tissue culture supernatant samples: ACTH, IL-6, OPG, Insulin, Leptin, DKK1, SOST, TNFα and FGF23. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Bone

Application

• Analytes: ACTH, DKK-1, FGF-23, IL-6, Insulin, Leptin, Osteoprotegerin (OPG), Sclerostin (SOST), TNF-α
• Recommended Sample Type: Mouse Serum, plasma, cell/tissue culture supernatants or lysates
• Recommended Sample Dilution: 25 μL per well of 1:2 diluted serum or plasma; cell/tissue culture samples may require dilution in appropriate control medium
• Assay Run Time: Overnight (16-18 hours) at 2-8°C or 2 hours at room temperature (20-25°C)
• Research Category: Bone
• Research Subcategory: Metabolism

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Other Notes

Please contact Technical Service for linearity of dilution.

Sensitivity: See kit protocol for individual analytes sensitivities.

Legal Information

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Información de seguridad

• pictograms: GHS06,GHS09
• signalword: Danger
• hcodes: H302 + H332,H311,H315,H317,H319,H335,H411
• pcodes: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects