Saltar al contenido
Merck
Todas las fotos(5)

Documentos

MABT397

Sigma-Aldrich

Anti-CEACAM1/CD66a Antibody, clone B3-17

clone B3-17, from mouse

Sinónimos:

Carcinoembryonic antigen-related cell adhesion molecule 1, Biliary glycoprotein 1, BGP-1, CD66a

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización


About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

B3-17, monoclonal

species reactivity

human

technique(s)

ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CEACAM1(634)

General description

CEACAM1 (carcinoembryonic antigen-related cell adhesion molecule 1) is thought to be a member of the immunoglobulin superfamily and is known as an epithelial tumor suppressor and an angiogenic growth factor. It has also been linked to the actin-based cytoskeleton. CEACAM1 is also known as a cellular receptor for a number of human mucosa pathogenic bacteria. The loss of activity of CEACAM1 has been related to the development of colorectal cancer.

Immunogen

Recombinant human CEACAM1/CD66a.

Application

ELISA Analysis: 10 µg/mL from a representative lot detected human CEACAM1/CD66a by ELISA (Courtesy of Dr. B. Singer, University Duisburg-Essen, Germany).

Flow Cytometry Analysis: 10 µg/mL from a representative lot detected the exogenously expressed human CEACAM1/CD66a on the surface of transfected HeLa cells (Courtesy of Dr. B. Singer, University Duisburg-Essen, Germany).

Immunocytochemistry Analysis: 10 µg/mL from a representative lot detected CEACAM1/CD66a on the surface of HT-29 human colorectal carcinoma cells (Courtesy of Dr. B. Singer, University Duisburg-Essen, Germany).

Immunohistochemistry Analysis: 10 µg/mL from a representative lot detected CEACAM1/CD66a immunoreactivity in human jejunum tissue (Courtesy of Dr. B. Singer, University Duisburg-Essen, Germany).

Western Blotting Analysis: 10 µg/mL from a representative lot detected the exogensously expressed human CEACAM1/CD66a extracellular domain-Fc fusion in lysates from transfected cells (Courtesy of Dr. B. Singer, University Duisburg-Essen, Germany).

Flow Cytometry Analysis: Representative lots, either unconjugated or FITC-conjugated, detected CECAM1 immunoreactivity on the surface of human peripheral blood naïve and memory B-cells (Khairnar, V., et al. (2015). Nat. Commun. 6:6217; Seifert, M., et al. (2015). Proc. Natl. Acad. Sci. 112(6):E546-555).

Flow Cytometry Analysis: A representative lot detected CEACAM1/CD66a induction on the surface of normal human bronchial epithelial (NHBE) cells stimulated with poly(I:C) or interferons (Klaile, E., et al. (2013) Respir Res. 14:85).

Flow Cytometry Analysis: A representative lot detected CEACAM1/CD66a expression on the surface of 2 day-starved human epithelial (HT29, T102/3) and endothelial (AS-M.5) cells, as well as multivesicular bodies (MVBs) derived from these cells (Muturi H.T., et al. (2013) PLoS One. 8(9):e74654).

Immunocytochemistry Analysis: A representative lot detected CEACAM1/CD66a immunoreactivity on the surface of normal human bronchial epithelial (NHBE) cells by indirect immunofluorescence staining (Klaile, E., et al. (2013) Respir Res. 14:85).

Immunohistochemistry Analysis: A representative lot detected CEACAM1/CD66a in paraffin-embedded human lung cancer tissue sections (Klaile, E., et al. (2013) Respir Res. 14:85).

Western Blot Analysis: A representative lot detected CEACAM1/CD66a in 2 day-starved AS-M.5 human endothelialn cells and AS-M.5-derived multivesicular bodies (MVBs) (Muturi H.T., et al. (2013) PLoS One. 8(9):e74654).
This Anti-CEACAM1/CD66a Antibody, clone B3-17 is validated for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, and Western Blotting for the detection of human CEACAM1/CD66a.

Quality

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected CEACAM1/CD66a in 10 µg of HepG2 cell lysate.

Target description

~160 kDa observed. Target band size appears larger than the calculated molecular weights of 57.56/53,80 kDa (pro-/mature isoform 1; BGPa, CEACAM1-4L, TM1-CEA), 45.95/42.19 kDa (pro-/mature isoform 2; BGPg, CEACAM1-4C1), 35.30/31.54 kDa (pro-/mature isoform 3; BGPh, CEACAM1-3), 38.58/34.81 kDa (pro-/mature isoform 4; BGPi, CEACAM1-3C2), 50.35/46.58 kDa (pro-/mature isoform 5; BGPy, CEACAM1-3AL), 46.91/43.15 kDa (pro-/mature isoform 6; BGPb, CEACAM1-3L, TM2-CEA), 27.43/23.67 kDa (pro-/mature isoform 7; BGPx, CEACAM1-1L), 50.52/46.76 kDa (pro-/mature isoform 8; BGPc, CEACAM1-4S, TM3-CEA), 43.06/39.30 kDa (pro-/mature isoform 9; BGPz, CEACAM1-3AS), 51.15/47.38 kDa (pro-/mature isoform 10), 39.87/36.11 kDa ((pro-/mature isoform 11; BGPd, CEACAM1-3S) due to glycosylation. Uncharacterized bands may be observed in some lysates.

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

Not finding the right product?  

Try our Herramienta de selección de productos.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Marc B Bechmann et al.
Oncotarget, 11(43), 3886-3899 (2020-11-17)
CEACAM5 is overexpressed in many primary breast carcinomas. However, the exact role of CEACAM5 in breast cancer tumorigenesis remains unresolved. Here, we examined a repository of 110 cryopreserved primary breast carcinomas by immunohistochemistry to assess the distribution of CEACAM5 in

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico