MABS1720

Anti-Acetyl-CoA Synthetase Antibody, clone 6C1.2

clone 6C1.2, from mouse

• Sinónimos: Acetyl-coenzyme A synthetase, cytoplasmic, Acetate-CoA ligase, Acetyl-CoA synthetase, AceCS, ACS, Acyl-activating enzyme, Acyl-CoA synthetase short-chain family member 2
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Propiedades

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 6C1.2, monoclonal
• species reactivity: human
• technique(s): western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_061147
• UniProt accession no.: Q9NR19
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... ACSS2(55902)

Descripción

General description

Acetyl-coenzyme A synthetase, cytoplasmic (EC 6.2.1.1; UniProt Q9NR19; also known as Acetate-CoA ligase, Acetyl-CoA synthetase, AceCS, ACS, Acyl-activating enzyme, Acyl-CoA synthetase short-chain family member 2) is encoded by the ACSS2 (also known as ACAS2) gene (Gene ID 55902) in human. ACS plays a vital role in prokaryotic and eukaryotic cells by activating acetate to form acetyl CoA that serves as a source of two carbon metabolite for lipid synthesis and energy producing reactions. It is also reported to play a role in gene expression. In yeast cells it delivers acetyl CoA for histone acetylation. ACS contributes to cancer cell growth under low-oxygen and lipid-depleted conditions and its expression is upregulated under metabolically stress. Silencing ACSS2 gene is shown to reduce the growth of tumor xenografts. Ref.: Starai, VJ., and Escalante-Semerena, JC. (2004). Cell. Mol. Life Sci. 61, 2020 2030. Schug, ZT et al. (2015). Cancer Cell. 27:57-71.

Specificity

Immunogen sequence is present in both spliced isoforms of human acetyl-CoA synthetase reported by UniProt (Q9NR19).

Immunogen

GST-tagged recombinant human acetyl-CoA synthetase N-terminal fragment.

Application

Detect Acetyl-CoA synthetase using this mouse monoclonal Anti-Acetyl-CoA Synthetase Antibody, clone 6C1.2, Cat. No. MABS1720, validated for use in Western Blotting.

Quality

Evaluated by Western Blotting in HEPG2 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected acetyl-CoA synthetase in 10 µg of HepG2 cell lysate.

Target description

~80 kDa observed. 78.58/80.09 kDa (isoform 1/2) calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

Información de seguridad

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable