MABN726

Anti-EphB2 Antibody, clone 2D12C6

ascites fluid, clone 2D12C6, from mouse

• Sinónimos: Ephrin type-B receptor 2, EPHB2 protein
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: ascites fluid
• tipo de anticuerpo: primary antibodies
• clon: 2D12C6, monoclonal
• reactividad de especies: rat, human
• técnicas: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotipo: IgG2b
• Nº de acceso UniProt: Q6NVW1
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... EPHB2(2048) , EphB2(2048) ; rat ... EphB2(313633)

Descripción

Descripción general

Ephrin type-B receptor 2 (EphB2) is a receptor tyrosine kinase for ephrin ligands that mediate numerous developmental processes, particularly in the nervous system. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB2 is critical for embryonic/postnatal development for lateral suprapyramidal blade formation which is in a specific region of the dentate gyrus. EphB2 is expressed in the two main components of the thymus, developing thymocytes, TEC, colorectal cancer, and in the earliest SGZ progenitor cells as they migrate during development and form the dentate gyrus.

Inmunógeno

Purified recombinant fragment of EphB2 expressed in E. coli.

Aplicación

Anti-EphB2 Antibody, clone 2D12C6 is a highly specific mouse monoclonal antibody, that targets EphB2 & has been tested in western blotting, IHC & ICC.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected EphB2 in HeLa and HepG2 cells.

Immunohistochemistry Analysis: 1:200 to 1:1,000 dilution from a representative lot detected EphB2 in rat cerebellum, pons/midbrain, and hippocampus tissues. Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Calidad

Evaluated by Western Blot in IMR-32 cell lysate.

Western Blot Analysis: A 1:500 dilution of this antibody detected EphB2 in 10 µg of IMR-32 cell lysate.

Descripción de destino

~53 kDa observed

Forma física

Mouse monoclonal IgG2b ascitic fluid containing up to 0.1% sodium azide.

Unpurified

Almacenamiento y estabilidad

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Nota de análisis

Control
IMR-32 cell lysate

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): nwg
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable